Simultaneous analysis of hydrodynamic and optical properties using analytical ultracentrifugation equipped with multiwavelength detection.
Simultaneous analysis of hydrodynamic and optical properties using analytical ultracentrifugation equipped with multiwavelength detection.
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DOI：
10.1021/ac504649c
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发表时间：
2015-03
影响因子：
7.4
通讯作者：
J. Walter;P. Sherwood;Wei Lin;D. Segets;W. Stafford;W. Peukert
中科院分区：
文献类型：
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作者：
J. Walter;P. Sherwood;Wei Lin;D. Segets;W. Stafford;W. Peukert
分析超速离心 (AUC) 已被证明是研究粒度分布、颗粒形状和相互作用的强大工具，具有高精度和未公开的分辨率。在这项工作中，我们展示了如何对配备多波长检测器 (MWL) 的 AUC 的沉降速度数据进行分析，以更深入地了解胶体和大分子混合物。新的数据评估例程已集成到软件 SEDAAL 中，以便处理 MWL 数据。这开辟了多种新的可能性，因为使用 MWL-AUC 同时可以获得混合物中各个成分的光谱信息。对于光学性质已知的系统，可以获取有关混合物中各个组分的流体动力学性质的信息。首次通过沉降速度数据的 MWL 评估来确定混合物中各组分的单独消光光谱。在我们的论文中，我们首先提供数据分析的信息背景，并公开我们方法的可访问参数。我们通过模拟数据进一步论证了数据评估。最后，我们给出了两个与纳米技术领域高度相关的例子，使用不同尺寸和消光特性的彩色二氧化硅和金纳米颗粒。
Analytical ultracentrifugation (AUC) has proven to be a powerful tool for the study of particle size distributions, particle shapes, and interactions with high accuracy and unrevealed resolution. In this work we show how the analysis of sedimentation velocity data from the AUC equipped with a multiwavelength detector (MWL) can be used to gain an even deeper understanding of colloidal and macromolecular mixtures. New data evaluation routines have been integrated in the software SEDANAL to allow for the handling of MWL data. This opens up a variety of new possibilities because spectroscopic information becomes available for individual components in mixtures at the same time using MWL-AUC. For systems of known optical properties information on the hydrodynamic properties of the individual components in a mixture becomes accessible. For the first time, the determination of individual extinction spectra of components in mixtures is demonstrated via MWL evaluation of sedimentation velocity data. In our paper we first provide the informational background for the data analysis and expose the accessible parameters of our methodology. We further demonstrate the data evaluation by means of simulated data. Finally, we give two examples which are highly relevant in the field of nanotechnology using colored silica and gold nanoparticles of different size and extinction properties.