Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development
Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development
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DOI：
10.1262/jrd.19096
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发表时间：
2007-12-01
影响因子：
1.8
通讯作者：
Tsunoda, Yukio
中科院分区：
文献类型：
--
作者：
Amarnath, Dasari;Li, Xiangping;Tsunoda, Yukio
由于供体核的异常表观遗传修饰而导致体细胞核移植（NT）胚胎中的异常基因表达可能是观察到的活力降低和发育异常的大部分原因。我们比较了由成年女性卵丘细胞和成年男性皮肤成纤维细胞产生的单个 8 细胞和囊胚期 NT 胚胎中 13 个发育重要基因的表达，新生儿异常发生率较低和较高 [1, 2]。体外受精（IVF）胚胎用作对照胚胎。在测试的基因中，Glut-1、IGF-IR、E-cad 和 Cx43 转录本的相对丰度在 8 细胞阶段的两种类型 NT 胚胎之间存在显着差异。与两种类型的 NT 胚胎相比，IVF 胚胎中锰超氧化物歧化酶 (MnSOD) 和 Stat3 转录物的相对丰度显着更高。在囊胚阶段，两种类型的NT胚胎之间只有一种基因Bcl-2的相对表达存在显着差异。尽管两种类型NT囊胚中Glut-1的表达水平没有差异，但其在两种类型NT囊胚中的表达均显着低于IVF囊胚中的表达。 NT 囊胚中的 MnSOD 表达水平往往较高。然而，任何单个基因的基因表达谱在个体胚胎之间变化很大，并且与胚胎形态无关。本研究表明，在具有不同新生儿异常发生率的两种不同类型供体细胞产生的第 9 天 NT 囊胚中检查的 13 个基因的表达谱在很大程度上无法区分。
Aberrant gene expression in somatic cell nuclear-transferred (NT) embryos due to abnormal epigenetic modifications of the donor nucleus likely accounts for much of the observed diminished viability and developmental abnormalities. We compared the expression of 13 developmentally important genes in individual 8-cell and blastocyst stage NT embryos produced from adults female cumulus cells and adult male skin fibroblast cells with low and high incidences of neonatal abnormalities [1, 2]. In vitro-fertilized (IVF) embryos were used as control embryos. Among the genes tested, the relative abundance of Glut-1, IGF-IR, E-cad, and Cx43 transcripts varied significantly between the two types of NT embryos at the 8-cell stage. The relative abundance of manganese super oxide dismutase (MnSOD) and Stat3 transcripts was significantly higher in IVF embryos compared with both types of NT embryos. At the blastocyst stage, there was a significant difference in the relative expression of only one gene, Bcl-2, between the two types of NT embryos. Although the level of Glut-1 expression did not vary between the two types of NT blastocysts, its expression in both types of NT blastocysts was significantly lower than that in IVF blastocysts. The MnSOD expression level tended to be higher in NT blastocysts. The gene expression profile for any single gene, however, was highly variable among individual embryos and was independent of embryo morphology. The present study demonstrated that the expression profiles of the 13 genes examined in Day 9 NT blastocysts produced from two different types of donor cells with different incidences of neonatal abnormalities are largely indistinguishable.