HELICOBACTER-FELIS GASTRITIS IN GNOTOBIOTIC-RATS - AN ANIMAL-MODEL OF HELICOBACTER-PYLORI GASTRITIS
HELICOBACTER-FELIS GASTRITIS IN GNOTOBIOTIC-RATS - AN ANIMAL-MODEL OF HELICOBACTER-PYLORI GASTRITIS
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DOI：
10.1128/iai.59.3.785-791.1991
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发表时间：
1991-03-01
影响因子：
3.1
通讯作者：
MURPHY, JC
中科院分区：
文献类型：
--
作者：
FOX, JG;LEE, A;MURPHY, JC
最初从猫胃中分离出的胃幽门螺杆菌（Helicobacter felis）在无菌大鼠的胃中定植。本文研究了无菌大鼠经口接种H.猫接种后2周，无菌大鼠胃粘膜中的淋巴细胞和嗜酸性粒细胞散在胃窦腺下区域的小病灶中。少量淋巴细胞存在于胃窦粘膜的腺下部分，其局部延伸穿过固有层朝向腔表面。接种后8周，炎症仅限于胃窦。其特征为腺下区域淋巴细胞和嗜酸性粒细胞数量增加，粘膜下层淋巴细胞局灶性聚集。一些淋巴聚集体从粘膜下层通过粘膜肌层和固有层延伸到腔表面。H.沃辛-斯塔里染色、细菌培养和尿素酶测定证实了猫，特别是在胃窦。H.猫也产生了显着的免疫球蛋白G抗体滴度在2，4，和8周接种后，以及一个短暂的免疫球蛋白M反应在2至4周接种后。接触对照组大鼠未感染，推测H.猫没有发生。
The gastric spirillum Helicobacter felis, originally isolated from the cat stomach, colonizes the stomachs of germfree rats. Studies were designed to examine the pathological and serological responses of germfree rats inoculated orally with H. felis. At 2 weeks postinoculation, the gastric mucosa of germfree rats had lymphocytes and eosinophils scattered in small foci throughout the subglandular region of the antrum. Small numbers of lymphocytes were present in the subglandular portion of the antral mucosa that focally extended through the lamina propria towards the luminal surface. Eight weeks postinoculation, the inflammation was confined to the antrum. It was characterized by increased numbers of lymphocytes and eosinophils in the subglandular areas, with focal aggregates of lymphocytes in the submucosa. Some lymphoid aggregates extended from the submucosa through the muscularis mucosa and lamina propria to the luminal surface. H. felis was demonstrated with the Warthin-Starry stain, bacterial culture, and urease assay, particularly in the antrum. H. felis also produced a significant immunoglobulin G antibody titer at 2, 4, and 8 weeks postinoculation as well as a transitory immunoglobulin M response at 2 to 4 weeks postinoculation. Contact control rats were not infected, inferring that fecal-oral spread of H. felis did not occur.