Polymeric electrospun scaffolds for bone morphogenetic protein 2 delivery in bone tissue engineering
Polymeric electrospun scaffolds for bone morphogenetic protein 2 delivery in bone tissue engineering
复制标题
用于骨组织工程中骨形态发生蛋白2递送的聚合物电纺支架
DOI：
10.1016/j.jcis.2018.07.029
复制
发表时间：
2018-12-01
影响因子：
9.9
通讯作者：
Mendoza, Gracia
中科院分区：
文献类型：
--
作者：
Aragon, Javier;Salerno, Simona;Mendoza, Gracia
假设：基于生物相容性聚合物的新型支架的开发在骨修复领域引起了极大的兴趣，以制备模拟细胞外基质并具有骨传导和骨诱导性能的可生物降解支架，以促进骨再生。实验：通过同时电纺和电喷涂的方法合成了聚己内酯(PCL)和聚己内酯/聚乙酸乙烯酯(PCL/PVAc)核壳纤维，并在其表面负载骨形态发生蛋白2(BMP2)的聚乳酸-乙醇酸[PLGA]微粒。将羟基磷灰石纳米棒(HAN)负载到纤维的核心。用扫描和透射电子显微镜、傅里叶变换红外光谱和热重分析对所得到的支架进行了表征。通过生物降解试验、成骨细胞活性测定以及碱性磷酸酶(ALP)、骨钙素(OCN)和骨桥蛋白(OPN)等特异性骨标志物的表达分析，评价这些材料的体外成骨能力。生长因子负载量为1.2-1.7微克/克支架，而HAN负荷量为8.8-12.6wt%。这些支架能够支持和促进细胞的生长和增殖，促进成骨和骨传导标志物(OCN和OPN)的表达。这些观察结果强调了BMP2在骨重建支架中的存在的重要性，以及新开发的支架在组织工程中临床应用的良好潜力。(C)2018 Elsevier Inc.保留所有权利。
Hypothesis: The development of novel scaffolds based on biocompatible polymers is of great interest in the field of bone repair for fabrication of biodegradable scaffolds that mimic the extracellular matrix and have osteoconductive and osteoinductive properties for enhanced bone regeneration.Experiments: Polycaprolactone (PCL) and polycaprolactone/polyvinyl acetate (PCL/PVAc) core-shell fibers were synthesised and decorated with poly(lactic-co-glycolic acid) [PLGA] particles loaded with bone morphogenetic protein 2 (BMP2) by simultaneous electrospinning and electrospraying. Hydroxyapatite nanorods (HAn) were loaded into the core of fibers. The obtained scaffolds were characterised by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The in vitro potential of these materials for bone regeneration was assessed in biodegradation assays, osteoblast viability assays, and analyses of expression of specific bone markers, such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN).Findings: PLGA particles were homogeneously distributed in the entire fibre mat. The growth factor load was 1.2-1.7 mu g/g of the scaffold whereas the HAn load was in the 8.8-12.6 wt% range. These scaffolds were able to support and enhance cell growth and proliferation facilitating the expression of osteogenic and osteoconductive markers (OCN and OPN). These observations underline the great importance of the presence of BMP2 in scaffolds for bone remodelling as well as the good potential of the newly developed scaffolds for clinical use in tissue engineering. (C) 2018 Elsevier Inc. All rights reserved.