Sec-independent protein translocation by the maize Hcf106 protein
Sec-independent protein translocation by the maize Hcf106 protein
复制标题
DOI：
10.1126/science.278.5342.1467
复制
发表时间：
1997-11-21
期刊：
影响因子：
56.9
通讯作者：
Martienssen, R
中科院分区：
文献类型：
--
作者：
Settles, AM;Yonetani, A;Martienssen, R
细菌 Sec 和信号识别颗粒（ffh 依赖性）蛋白质易位机制在原核生物和高等植物叶绿体之间是保守的。叶绿体中的第三种易位机制[质子浓度差（Delta pH）途径]以前被认为是独特的。玉米的 hcf106 突变破坏了分离叶绿体中通过 Delta pH 途径转运的蛋白质的定位。 Hcf106基因编码一种类似受体的类囊体膜蛋白，该蛋白与所有完全测序的细菌基因组的开放阅读框具有同源性，这表明自叶绿体内共生起源以来，Delta pH途径一直是保守的。因此，在细菌和植物中都发现了第三种蛋白质易位途径（HCF106 是其中的一个组成部分）。
The bacterial Sec and signal recognition particle (ffh-dependent) protein translocation mechanisms are conserved between prokaryotes and higher plant chloroplasts. A third translocation mechanism in chloroplasts [the proton concentration difference (Delta pH) pathway] was previously thought to be unique. The hcf106 mutation of maize disrupts the localization of proteins transported through this Delta pH pathway in isolated chloroplasts. The Hcf106 gene encodes a receptor-like thylakoid membrane protein, which shows homology to open reading frames from all completely sequenced bacterial genomes, which suggests that the Delta pH pathway has been conserved since the endosymbiotic origin of chloroplasts. Thus, the third protein translocation pathway, of which HCF106 is a component, is found in both bacteria and plants.