Multilayer Paper-Based Device for Colorimetric and Electrochemical Quantification of Metals
Multilayer Paper-Based Device for Colorimetric and Electrochemical Quantification of Metals
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DOI：
10.1021/ac5000224
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发表时间：
2014-04-01
影响因子：
7.4
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
--
作者：
Rattanarat, Poomrat;Dungchai, Wijitar;Henry, Charles S.
金属和含金属化合物释放到环境中越来越受到发达国家和发展中国家的关注，因为人类接触金属几乎会对每个器官系统产生不利的健康影响。不幸的是，量化环境中的金属成本很高。使用经过认证的实验室进行分析的成本通常超过 100 美元/样品，这使得有毒金属的常规分析对于职业暴露或环境保护等应用来说成本过高。在这里，我们报告了一种简单、廉价的技术，该技术有可能使发展中国家和发达国家都能进行有毒金属检测，该技术将比色法和电化学微流体纸基分析装置 (mPAD) 结合在三维配置中。与之前设计用于测量金属的 mPAD 不同，此处报道的设备将一层上的比色检测与另一层上的电化学检测分开。单独的检测层允许将不同的化学物质应用于同一设备上的单个样品。为了证明该方法的有效性，对 Ni、Fe、Cu 和 Cr 进行了比色检测，对 Pb 和 Cd 进行了电化学检测。比色层的检测限低至 0.12 μg（Cr），而电化学层的检测限低至 0.25 ng（Cd 和 Pb）。证明了目标分析物对常见干扰的选择性。作为设备实用程序的一个示例，对空气采样过滤器上收集的颗粒金属进行了分析。使用 mPAD 测量的水平与收集的颗粒物的经过认证的参考样品的已知值相匹配。
The release of metals and metal-containing compounds into the environment is a growing concern in developed and developing countries, as human exposure to metals is associated with adverse health effects in virtually every organ system. Unfortunately, quantifying metals in the environment is expensive; analysis costs using certified laboratories typically exceed $100/sample, making the routine analysis of toxic metals cost-prohibitive for applications such as occupational exposure or environmental protection. Here, we report on a simple, inexpensive technology with the potential to render toxic metals detection accessible for both the developing and developed world that combines colorimetric and electrochemical microfluidic paper-based analytical devices (mPAD) in a three-dimensional configuration. Unlike previous mPADs designed for measuring metals, the device reported here separates colorimetric detection on one layer from electrochemical detection on a different layer. Separate detection layers allows different chemistries to be applied to a single sample on the same device. To demonstrate the effectiveness of this approach, colorimetric detection is shown for Ni, Fe, Cu, and Cr and electrochemical detection for Pb and Cd. Detection limits as low as 0.12 mu g (Cr) were achieved on the colorimetric layer while detection limits as low as 0.25 ng (Cd and Pb) were achieved on the electrochemical layer. Selectivity for the target analytes was demonstrated for common interferences. As an example of the device utility, particulate metals collected on air sampling filters were analyzed. Levels measured with the mPAD matched known values for the certified reference samples of collected particulate matter.