Evaluation of dermal wound healing activity of synthetic peptide SVVYGLR
Evaluation of dermal wound healing activity of synthetic peptide SVVYGLR
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合成肽SVVYGLR的真皮伤口愈合活性评价
DOI：
10.1016/j.bbrc.2017.07.124
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发表时间：
2017
影响因子：
3.1
通讯作者：
Yamamoto Hirofumi
中科院分区：
文献类型：
--
作者：
Uchinaka Ayako;Kawaguchi Naomasa;Ban Tsuyoshi;Hamada Yoshinosuke;Mori Seiji;Maeno Yoshitaka;Sawa Yoshiki;Nagata Kohzo;Yamamoto Hirofumi
SVVYGLR肽（SV peptide）是一种来源于细胞外基质中骨桥蛋白的具有血管生成活性的7个氨基酸序列，可促进成纤维细胞向肌纤维母细胞样细胞分化，并促进心脏成纤维细胞产生Ⅲ型胶原。然而，SV肽对真皮细胞和组织的影响尚不清楚。在这项研究中，我们评估了这种肽在大鼠皮肤伤口愈合模型中的作用。将合成的SV肽加入到真皮成纤维细胞或角质形成细胞中，并评价它们的细胞运动性。在体内伤口愈合实验中，将8周龄的雄性大鼠随机分配到SV肽治疗组、未治疗对照组或磷酸盐缓冲盐水（PBS）组。通过创面修复率和组织学特征评价创面愈合情况。划痕实验和趋化细胞迁移实验表明，SV肽能显著促进成纤维细胞和角质形成细胞的迁移。相反，这些细胞的增殖能力不受SV肽的影响。在大鼠模型中，SV肽治疗组的伤口愈合进展快于对照组和PBS组。组织病理学分析显示SV肽处理刺激成纤维细胞向伤口区域的迁移并增加肌成纤维细胞的数量。免疫组织化学染色显示SV肽处理组中血管性血友病因子阳性的新微血管显著增加。总之，SV肽具有通过刺激血管生成、细胞迁移和成纤维细胞的成肌纤维细胞分化来刺激肉芽形成以促进伤口愈合的有益功能。
SVVYGLR peptide (SV peptide) is a 7-amino-acid sequence with angiogenic properties that is derived from osteopontin in the extracellular matrix and promotes differentiation of fibroblasts to myofibroblast-like cells and the production of collagen type Ⅲ by cardiac fibroblasts. However, the effects of SV peptide on dermal cells and tissue are unknown. In this study, we evaluated the effects of this peptide in a rat model of dermal wound healing. The synthetic SV peptide was added to dermal fibroblasts or keratinocytes, and their cellular motility was evaluated. In an in vivo wound healing exeriment, male rats aged 8 weeks were randomly assigned to the SV peptide treatment, non-treated control, or phosphate-buffered saline (PBS) groups. Wound healing was assessed by its repair rate and histological features. Scratch assay and cell migration assays using the Chemotaxicell method showed that SV peptide significantly promoted the cell migration in both fibroblasts and keratinocytes. In contrast the proliferation potency of these cells was not affected by SV peptide. In the rat model, wound healing progressed faster in the SV peptide-treated group than in the control and PBS groups. The histopathological analyses showed that the SV peptide treatment stimulated the migration of fibroblasts to the wound area and increased the number of myofibroblasts. Immunohistochemical staining showed a marked increase of von Willebland factor-positive neomicrovessels in the SV peptide-treated group. In conclusion, SV peptide has a beneficial function to promote wound healing by stimulating granulation via stimulating angiogenesis, cell migration, and the myofibroblastic differentiation of fibroblasts.