SENSITIBITY OF VARIOUS METHODS IN THE DETECTION OF IgG ANTIBODIES TO RED CELL ANTIGENS
SENSITIBITY OF VARIOUS METHODS IN THE DETECTION OF IgG ANTIBODIES TO RED CELL ANTIGENS
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检测红细胞抗原 IgG 抗体的各种方法的敏感性
DOI：
10.3925/jjtc1958.49.640
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
N. Watanabe
中科院分区：
文献类型：
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作者：
M. Sasaki;M. Yamada;R. Kaneko;D. Furuya;T. Endoh;D. Kobayashi;N. Tsuji;A. Yagihashi;N. Watanabe
近年来，使用自动化仪器的微管柱凝集法和微孔板法等新方法已用于血型分型、抗体筛选试验和交叉配血试验。然而，没有精确的比较研究的性能，这些方法和传统的管方法已经完成。在本研究中，我们通过使用聚乙二醇（PEG）、白蛋白或低离子强度盐溶液（利斯）的试管间接抗球蛋白试验（达特）、微管柱凝集（Ortho BioVue和Dialled MTS）和微孔板固相系统（Immucor Capture R Ready Screen，CRRS）方法对IgG同种抗体的灵敏度进行了比较研究。用TAT-PEG法检测51份阳性血清，TAT-LISS法、BioVue法、MTS法和CRRS法的阳性率分别为62.7%、68.6%、88.2%、88.2%和94.1%。用12份市售抗血清和25份病人血清，比较了这6种方法之间的抗体反应性（用AABB评分测定）。TATPEG试管的平均AABB评分为51.4，TAT-白蛋白试管为37.3，TAT-LISS试管为37.4，BioVue试管为51.7，MTS试管为53.8，CRRS试管为76.5。这些结果表明，具有临床意义的红细胞同种抗体的灵敏度与微管柱凝集法和CRRS相似或高于试管达特法。
In recent years, new methods such as microtube column agglutination methods and microplate methods using automated instruments have been used for blood typing, antibody screening testing and crossmatching testing. However, no precise comparison study of the performance of these methods and conventional tube methods has been done. In the present study, we performed a comparison study on sensitivity for IgG alloantibody by the tube indirect anti-globulin test (TAT) using polyethylene glycol (PEG), albumin, or low ionic strength saline solution (LISS), microtube column agglutination (Ortho BioVue and Dialled MTS), and microplate solid phase system (Immucor Capture R Ready Screen, CRRS) methods. Using 51 positive sera as determined by the tube TAT-PEG using anti-IgG antibody, the positive rates of tube TAT-albumin and tube TAT-LISS using anti-IgG antibody, BioVue (IgG cassette), MTS (IgG card) and CRRS (IgG bonded RBC) were 62.7%, 68.6%, 88.2%, 88.2% and 94.1%, respectively. Using 12 commercial anti-sera and 25 patient sera, antibody reactivity as determind by AABB scores was compared between these 6 methods. Mean AABB scores were 51.4 with the tube TATPEG, 37.3 with the tube TAT-albumin, 37.4 with the tube TAT-LISS, 51.7 with BioVue, 53.8 with MTS, and 76.5 with CRRS. These results indicate that the sensitivity of clinically significant red cell alloantibodies was similar or higher with the microtube column agglutination methods and CRRS than with the tube TAT methods.