Human apolipoprotein B: partial amino acid sequence.
Human apolipoprotein B: partial amino acid sequence.
复制标题
人载脂蛋白B：部分氨基酸序列。
DOI：
10.1016/0014-5793(84)81378-2
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发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
Lusis,AJ
中科院分区：
文献类型：
--
作者：
LeBoeuf,RC;Miller,C;Shively,JE;Schumaker,VN;Balla,MA;Lusis,AJ
根据Cleveland等的方法[（1977）J.Biol.Chem.252，1102-1106]，已经开发出一种成功的方法用于对载脂蛋白B进行测序，该方法涉及在十二烷基硫酸钠存在下的有限蛋白水解。使用旋转杯微测序技术对两种肽进行部分测序。序列为：肽R2 - 5，-Ala-Leu-瓦尔-Gly-Ile-Asn-Gly-Glu-Ala-Asn-Leu-Asp-Phe-Leu-Asn-Ile-Pro-Leu-Arg-Ile-Pro-Pro-Met-Arg-（Arg）-;和肽R3 - 1，-Leu-瓦尔-Ala-Lys-Pro-Ser-瓦尔-Ser-瓦尔-Glu-Phe-瓦尔-Thr-Asn-Met-Gly-Ile-Ile-Pro-Lys-Phe-Ala-Arg-。已经鉴定了适合于构建寡核苷酸探针的几段残基。
A successful approach has been developed for the sequencing of apolipoprotein B based upon the procedure of Cleveland et al. [(1977) J. Biol. Chem. 252, 1102–1106] involving limited proteolysis in the presence of sodium dodecyl sulfate.Staphylococcus aureusprotease was employed to produce large peptides which were isolated in relatively pure form by preparative gel electrophoresis. Two peptides were partially sequenced using spinning‐cup microsequencing techniques. The sequences are: Peptide R2‐5, ‐Ala ‐ Leu ‐ Val ‐ Gly ‐ Ile ‐ Asn ‐ Gly ‐ Glu ‐ Ala ‐ Asn ‐ Leu ‐ Asp ‐ Phe ‐ Leu ‐ Asn ‐ Ile ‐ Pro ‐ Leu ‐ Arg‐ Ile ‐ Pro‐ Pro‐Met‐Arg‐(Arg)‐; and Peptide R3‐1, ‐Leu‐Val‐Ala‐Lys‐Pro‐Ser‐Val‐Ser‐Val‐Glu‐Phe‐Val‐Thr ‐Asn‐Met‐Gly‐Ile‐Ile‐Ile‐Pro‐Lys‐Phe‐Ala‐Arg‐. Several stretches of residues suitable for the construction of oligonucleotide probes have been identified.