Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wall.
Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wall.
复制标题
过量的质膜和离子两性分子对外毛细胞侧壁力学的影响。
DOI：
10.1152/ajpcell.00210.2001
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Brownell,WilliamE
中科院分区：
文献类型：
--
作者：
Morimoto,Noriko;Raphael,RobertM;Nygren,Anders;Brownell,WilliamE
外毛细胞（OHC）侧壁质膜和下面的皮质晶格之间的相互作用进行了检查细胞变形过程中的细胞图像的形态计量学分析。通过微量吸管抽吸对照细胞和暴露于改变膜力学的离子两性分子的细胞，产生质膜的囊泡化。总细胞和囊泡表面积的增加表明，质膜具有膜水库。氯丙嗪（CPZ）降低所需的压力，而水杨酸盐（Sal）没有影响。CPZ降低了囊泡形成所需的时间，表明CPZ降低了囊泡形成所需的能垒。在微量移液管抽吸期间观察到总体积增加。变形引起的水力传导性的增加也被视为响应于微量移液管施加的流体射流的侧壁变形。CPZ和/或Sal的应用降低了这种应变引起的水力传导度。离子两亲分子对OHC质膜和侧壁力学的影响可能有助于其对OHC电活动性和听力的影响。
The interaction between the outer hair cell (OHC) lateral wall plasma membrane and the underlying cortical lattice was examined by a morphometric analysis of cell images during cell deformation. Vesiculation of the plasma membrane was produced by micropipette aspiration in control cells and cells exposed to ionic amphipaths that alter membrane mechanics. An increase of total cell and vesicle surface area suggests that the plasma membrane possesses a membrane reservoir. Chlorpromazine (CPZ) decreased the pressure required for vesiculation, whereas salicylate (Sal) had no effect. The time required for vesiculation was decreased by CPZ, indicating that CPZ decreases the energy barrier required for vesiculation. An increase in total volume is observed during micropipette aspiration. A deformation-induced increase in hydraulic conductivity is also seen in response to micropipette-applied fluid jet deformation of the lateral wall. Application of CPZ and/or Sal decreased this strain-induced hydraulic conductivity. The impact of ionic amphipaths on OHC plasma membrane and lateral wall mechanics may contribute to their effects on OHC electromotility and hearing.