Rapid Determination of Volatile Compounds Emitted from Chimonanthus praecox Flowers by HS-SPME-GC-MS
Rapid Determination of Volatile Compounds Emitted from Chimonanthus praecox Flowers by HS-SPME-GC-MS
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DOI：
10.1515/znc-2004-9-1005
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发表时间：
2004-10
期刊：
影响因子：
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通讯作者：
Chunhui Deng;Guoxin Song;Yaoming Hu
中科院分区：
文献类型：
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作者：
Chunhui Deng;Guoxin Song;Yaoming Hu
摘要采用顶空固相微萃取（HS-SPME）和气相色谱-质谱（GC-MS）联用技术，建立了一种快速、简便、无溶剂的蜡梅花挥发性成分分析方法。首次优化了固相微萃取条件，并将其应用于蜡梅活体挥发性成分的分析。花和离体蜡梅。花首次鉴定出31种化合物，主要包括4-甲基-1，3-戊二烯（2.0%）、α-水芹烯（4.7%）、苄基甲醇（11.1%）、反式芳樟醇氧化物（呋喃环）（5.3%）、α-芳樟醇（36.0%）、水杨酸甲酯（24.5%）和乙酸苄酯（5.9%）。通过比较活体和离体花的排放物，共检测到28种化合物，其中只有正十五烷、正十六烷和正十七烷3种化合物在活体花中检测到，表明它们可能是生物标志物。
Abstract A novel rapid, simple and solvent-free method was developed for determination of the volatile compounds from the flowers of Chimonanthus praecox Link using headspace solidphase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The SPME conditions were firstly optimized and applied to sampling of the volatile compounds emitted from living Chimonanthus praecox L. flowers and excised Chimonanthus praecox L. flowers. Thirty-one compounds emitted from living flowers were identified for the first time, which mainly included 4-methyl-1,3-pentadiene (2.0%), α-phellandrene (4.7%), benzyl methanol (11.1%), trans-linalool oxide (furanyl ring) (5.3%), α-linalool (36.0%), methyl salicylate (24.5%) and acetic acid benzyl ester (5.9%). Comparing the emission from living flowers and excised flowers, twenty-eight compounds were found to be detected in the two emissions, and three compounds, n-pentadecane, n-cetane and n-heptadecane, were only found in the emission from the living flowers, which shows that they might be biomarker compounds.