THERE ARE 2 MAJOR TYPES OF HEPATITIS-C VIRUS IN JAPAN
THERE ARE 2 MAJOR TYPES OF HEPATITIS-C VIRUS IN JAPAN
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DOI：
10.1016/0006-291x(90)90494-8
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发表时间：
1990-08-16
影响因子：
3.1
通讯作者：
DATE, T
中科院分区：
文献类型：
--
作者：
ENOMOTO, N;TAKADA, A;DATE, T
应用聚合酶链反应（PCR）检测慢性非甲、非乙型肝炎患者血浆丙型肝炎病毒（HCV）。通过选择合适的引物，24份样本中有19份（79%）呈阳性。对编码NS5部分基因的400 bp cDNA扩增片段的序列分析表明，日本HCV可分为两种类型（K1和K2）。槽点杂交显示，13个片段为HCV-K1, 6个片段为HCV-K2，与原型核苷酸序列同源性分别为80%和67%。
The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene suggested that HCV can be classified into two types (named K1 and K2) in Japan. Slot blot hybridization of the fragments indicated that 13 were HCV-K1 and 6 were HCV-K2, which show 80% and 67% nucleotide sequence homology, respectively, with that of the prototype.