Transcriptome-wide Investigation of mRNA/circRNA in miR-184 and Its r.57c > u Mutant Type Treatment of Human Lens Epithelial Cells.
Transcriptome-wide Investigation of mRNA/circRNA in miR-184 and Its r.57c > u Mutant Type Treatment of Human Lens Epithelial Cells.
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miR-184 及其 r.57c > u 人晶状体上皮细胞突变型治疗中 mRNA/circRNA 的全转录组研究
DOI：
10.1016/j.omtn.2017.02.008
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发表时间：
2017-06-16
期刊：
影响因子：
--
通讯作者：
Yao K
中科院分区：
文献类型：
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作者：
Luo Y;Liu S;Yao K
m-miR-184（突变体miR-184，r.57c > u）出现在家族性遗传性眼部疾病中，包括圆锥角膜、白内障、EDICT（内皮营养不良、虹膜发育不全、先天性白内障和基质变薄）综合征、严重圆锥角膜和非扩张性角膜变薄。m-miR-184在这些眼部疾病中的生物学功能仍不清楚。随着高通量测序的出现，现在可以同时发现许多不同的生物成分。使用两种不同的RNA文库，我们对用miR-184、m-miR-184和阴性对照处理的HLE细胞的完整转录组进行测序。整合数据以鉴定受m-miR-184影响的任何新基因。值得注意的是，我们得出结论，ALDH 5A 1和GABRA 3被m-miR-184扰乱，这可能导致眼部疾病。此外，在miR-184、m-miR-184和阴性对照处理组中，circRNA（环状RNA）表达是高度随机的。circRNA的序列确实揭示了特别高水平的ALU序列。总之，我们为理解m-miR-184在眼部疾病中的作用提供了新的途径。
m-miR-184 (mutant miR-184, r.57c > u) appears in familial hereditary ocular diseases, including keratoconus, cataracts, EDICT (endothelial dystrophy, iris hypoplasia, congenital cataract, and stromal thinning) syndrome, severe keratoconus, and non-ectatic corneal thinning. The biological function of m-miR-184 in these ocular diseases remains unclear. With the emergence of high-throughput sequencing, it is now possible to discover many different biological components simultaneously. Using two different RNA libraries, we sequenced the complete transcriptome of HLE cells treated with miR-184, m-miR-184, and a negative control. Data were integrated in an effort to identify any novel gene affected by m-miR-184. Notably, we concluded that ALDH5A1 and GABRA3 were disordered by m-miR-184, which might lead to ocular disease. Moreover, circRNA (circular RNA) expression was highy random across miR-184, m-miR-184, and negative control treatment groups. The sequences of the circRNAs did reveal a particularly high level of ALU sequences. In summary, we provide a new avenue for understanding the role of m-miR-184 in ocular diseases.