Synaptic connections and small circuits involving excitatory and inhibitory neurons in layers 2-5 of adult rat and cat neocortex:: Triple intracellular recordings and biocytin labelling in vitro
Synaptic connections and small circuits involving excitatory and inhibitory neurons in layers 2-5 of adult rat and cat neocortex:: Triple intracellular recordings and biocytin labelling in vitro
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DOI：
10.1093/cercor/12.9.936
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发表时间：
2002-09-01
期刊：
影响因子：
3.7
通讯作者：
Bannister, AP
中科院分区：
文献类型：
--
作者：
Thomson, AM;West, DC;Bannister, AP
在成人新皮层切片中用生物胞素标记的双重和三重细胞内记录探索了突触连接神经元的小回路。大鼠679对和猫319对分别记录到135和42个兴奋性突触后电位（EPSP）和37和26个抑制性突触后电位（IPSP）。模式的连接和突触特性是相似的，在这两个物种，虽然规模和形态的范围内的差异进行了观察。兴奋性的“向前”的预测从第4层到第3层，像那些从第3层到第5层，针对锥体细胞和一小部分的中间神经元，而兴奋性的“向后”的预测从第3层到第4层选择的中间神经元，包括小白蛋白免疫阳性篮细胞。抑制第3层锥体细胞的第4层中间神经元包括篮状细胞和树突靶向细胞。大的中间神经元，类似于细胞先前描述为大篮细胞，在第4层和第3层（猫），长有髓鞘的水平轴突侧支接受频繁的兴奋性输入从两个层。一个非常高的连接率之间的interneurons，往往有相当不同的形态，并由此产生的IPSPs，如记录在interneurons的EPSPs，是简短的相比，记录在锥体和多刺的星状细胞。
Dual and triple intracellular recordings with biocytin labelling in slices of adult neocortex explored small circuits of synaptically connected neurons. 679 paired recordings in rat and 319 in cat yielded 135 and 42 excitatory postsynaptic potentials (EPSPs) and 37 and 26 inhibitory postsynaptic potentials (IPSPs), respectively. Patterns of connectivity and synaptic properties were similar in the two species, although differences of scale and in the range of morphologies were observed. Excitatory 'forward' projections from layer 4 to 3, like those from layer 3 to 5, targeted pyramidal cells and a small proportion of interneurons, while excitatory 'back' projections from layer 3 to 4 selected interneurons, including parvalbumin immuno-positive basket cells. Layer 4 interneurons that inhibited layer 3 pyramidal cells included both basket cells and dendrite-targeting cells. Large interneurons, resembling cells previously described as large basket cells, in layers 4 and 3 (cat), with long myelinated horizontal axon collaterals received frequent excitatory inputs from both layers. A very high rate of connectivity was observed between pairs of interneurons, often with quite different morphologies, and the resultant IPSPs, like the EPSPs recorded in interneurons, were brief compared with those recorded in pyramidal and spiny stellate cells.