Tyrosine hydroxylase and neuropeptide-Y immunoreactivity in pineal glands developing in situ and in pineal grafts.
Tyrosine hydroxylase and neuropeptide-Y immunoreactivity in pineal glands developing in situ and in pineal grafts.
复制标题
原位发育的松果体和松果体移植物中的酪氨酸羟化酶和神经肽-Y 免疫反应性。
DOI：
10.1007/bf00319041
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发表时间：
1991
影响因子：
3.6
通讯作者：
Welsh,MG
中科院分区：
文献类型：
--
作者：
Li,K;Welsh,MG
使用亲和素-生物素-过氧化物酶免疫组织化学技术检查松果体原位神经支配的出生后发育以及酪氨酸羟化酶（TH）和神经肽Y（NPY）免疫反应性神经纤维对松果体移植物的再神经支配。仓鼠和沙鼠出生后第二天（P2）的松果体中出现了 TH 免疫反应性神经纤维。 NPY 免疫反应性神经纤维首先在 P2 时出现在沙鼠的松果体中，在 P3 时出现在仓鼠中。到出生后第七天（P7），仓鼠和沙鼠的松果体都受到 TH 纤维和 NPY 纤维的丰富支配，这些纤维表现为光滑纤维或具有偶发性静脉曲张的纤维。到 4 周大时，仓鼠和沙鼠松果体的 TH 纤维和 NPY 纤维的神经支配已完全发育。丰富的 TH 纤维和 NPY 纤维在浅层和深层松果体的薄壁组织中形成致密的网状结构。绝大多数纤维具有大量的静脉曲张。在沙鼠的松果体中发现的 NPY 纤维比仓鼠更多。 NPY纤维均匀分布在沙鼠的整个松果体中，但它们更常见于仓鼠浅表松果体的中央区域。对于松果体移植物，将新生和4周龄仓鼠的浅表松果体移植到第三脑室的不同部位（漏斗状隐窝、第三脑室后部）或肾囊下方。来自 4 周大捐赠者的松果体移植物似乎经历了严重的退化并最终消失。然而，来自新生仓鼠的松果体移植物成功存活并很好地融入了新的位置。在第三脑室的松果体移植物周围发现了宿主大脑中丰富的 TH 和 NPY 纤维，但很少看到进入移植物的实质。移植后4周，在肾移植物中发现了一些TH-纤维。这些研究描述了 TH 和 NPY 神经纤维原位松果体神经支配的出生后发育，并证明了来自邻近宿主大脑的 TH 和 NPY 纤维对脑室松果体移植物的重新神经支配。
Postnatal development of the innervation of the pineal gland in situ as well as the reinnervation of pineal grafts by tyrosine hydroxylase (TH)- and neuropeptide Y (NPY)-immunoreactive nerve fibers were examined using the avidin-biotin-peroxidase immunohistochemical technique. TH-immunoreactive nerve fibers appeared in the pineal gland on the second postnatal day (P2) in both hamsters and gerbils. NPY-immunoreactive nerve fibers first appeared in the pineal gland of gerbils on P2 and in the hamsters on P3. By the seventh postnatal day (P7), the pineal glands of both hamsters and gerbils were richly innervated by TH- and NPY-fibers that appeared as smooth fibers or fibers with sporadic varicosities. By the age of 4 weeks, the innervation of the pineal glands of hamsters and gerbils by TH-and NPY-fibers was fully developed. Abundant TH- and NPY-fibers formed a dense meshwork in the parenchyma of the superficial and deep pineals. The great majority of the fibers bore a large number of varicosities. More NPY-fibers were found in the pineal glands of gerbils than hamsters. NPY fibers were distributed evenly throughout the pineal glands of the gerbil, but they were more often located in the central region of the superficial pineal of the hamster. For the pineal grafts, superficial pineals from neonatal and 4-week-old hamsters were transplanted to different sites in the third cerebral ventricle (infundibular recess, posterior third ventricle) or beneath the renal capsule. The pineal grafts from 4-week-old donors appeared to undergo severe degeneration and eventually disappeared. The pineal grafts from neonatal hamsters, however, successfully survived and became well integrated into their new locations. Abundant TH-and NPY-fibers in the host brain were found surrounding the pineal grafts placed in the third cerebral ventricle, but were only rarely seen entering the parenchyma of the grafts. A few TH-fibers were demonstrated in the renal grafts 4 weeks after transplantation. These studies describe the postnatal development of the innervation of the pineal glands in situ by TH-and NPY-nerve fibers, and demonstrate a lack of reinnervation of cerebroventricular pineal grafts by TH and NPY fibers from adjacent host brain.