Wnt and Notch signaling pathways in calcium phosphate-enhanced osteogenic differentiation: A pilot study
Wnt and Notch signaling pathways in calcium phosphate-enhanced osteogenic differentiation: A pilot study
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磷酸钙增强成骨分化中的 Wnt 和 Notch 信号通路：一项初步研究
DOI：
10.1002/jbm.b.34105
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发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Li, Jihua
中科院分区：
文献类型：
--
作者：
Wang, Yu;Pan, Jianwei;Li, Jihua
磷酸钙陶瓷因其生物相容性、生物安全性、多孔结构和骨诱导性等优点，越来越多地被用于修复严重骨缺损。Wnt和Notch信号通路是调控骨髓间充质干细胞（BMSCs）成骨分化的经典信号通路。本研究采用实时荧光定量PCR（real-time RT-PCR）技术，检测了Wnt信号通路抑制剂DKK 1对BMSCs在CaP陶瓷上成骨相关基因表达的影响。此外，我们还评估了体内植入CaP陶瓷后的新骨形成。体外培养显示Wnt、Notch信号通路及成骨相关基因的表达呈先升高后降低的趋势。加入DKK 1后，表达下降。体内植入显示CaP陶瓷上有明显的骨形成。这些结果表明Wnt和Notch信号通路在CaP促进成骨分化中发挥重要作用，但其协同作用机制有待进一步探讨。(c)2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，107 B：149-160，2019。
Calcium phosphate (CaP) ceramics are being increasingly used to repair critical bone defect for biocompatibility, biosafety, porous structure and osteoinduction. Wnt and Notch signaling pathways are conservative and classic signaling pathways regulating osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSCs). In this study, we evaluated the expression of Wnt, Notch signal pathways and osteogenic related genes of BMSCs with and without Wnt pathway inhibitor DKK1 on CaP ceramics in vitro by real-time RT-PCR. Also, we evaluated new bone formation after implantation of CaP ceramics in vivo. The in vitro culture displayed the expression of Wnt, Notch signaling pathways and osteogenic related genes was increased at first and then declined. After adding DKK1 to the culture, the expression was decreased. The in vivo implantation showed significant bone formation on CaP ceramics. These findings reveal that Wnt and Notch signaling pathways play important roles in the CaP-enhanced osteogenic differentiation but the mechanism of synergistic effect needs to be further explored. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 149-160, 2019.