Tumor-Associated Macrophages Associate with Cerebrospinal Fluid Interleukin-10 and Survival in Primary Central Nervous System Lymphoma (PCNSL)
Tumor-Associated Macrophages Associate with Cerebrospinal Fluid Interleukin-10 and Survival in Primary Central Nervous System Lymphoma (PCNSL)
复制标题
肿瘤相关巨噬细胞与脑脊液白细胞介素 10 和原发性中枢神经系统淋巴瘤 (PCNSL) 生存相关
DOI：
10.1111/bpa.12318
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kohmura E
中科院分区：
文献类型：
--
作者：
Sasayama T;Tanaka K;Mizowaki T;Nagashima H;Nakamizo S;Tanaka H;Nishihara M;Mizukawa K;Hirose T;Itoh T;Kohmura E
据报道，肿瘤相关巨噬细胞（TAM）增加与各种肿瘤的预后不良相关;然而，TAM在原发性中枢神经系统淋巴瘤（PCNSL）中的重要性尚未阐明。在47例接受大剂量甲氨蝶呤（MTX）和放疗的PCNSL患者中，分析了TAM浸润水平与临床病理参数之间的关系。使用连续量表的考克斯比例风险模型的单变量分析显示，CD 68阳性（+）TAM增加与无进展生存期（PFS）较低显著相关（P= 0.04），观察到CD 163 + TAM增加和PFS较短的趋势（P= 0.05）。然而，TAM增加与总生存期无关。因为已知TAM产生各种细胞因子，我们研究了脑脊液（CSF）细胞因子和TAM之间的关系。CSF中IL-6和可溶性IL-2受体与TAM的浸润率无关;但CSF中IL-10水平与CD 68和CD 163 + TAM的浸润水平相关。我们还通过双重免疫染色分析证实了IL-10在CD 68+和CD 163 + TAM中的表达。我们的结果表明，CSF中高水平的IL-10可能与PCNSL中TAM的浸润水平呈正相关。
Increased tumor‐associated macrophages (TAMs) have been reported to be associated with poor prognosis in various tumors; however, the importance of TAMs in primary central nervous system lymphoma (PCNSL) has not been clarified. In 47 patients with PCNSL who were treated with high‐dose methotrexate (MTX) and radiotherapy, the relationships between the infiltration levels of TAMs and the clinicopathological parameters were analyzed. Univariate analysis of the Cox proportional hazards model using continuous scales revealed that increased CD68 positive (+) TAMs was significantly associated with inferior progression‐free survival (PFS) (P= 0.04), and trends were observed for the increased CD163+TAMs and having shorter PFS (P= 0.05). However, increased TAMs were not associated with overall survival. Because TAMs are known to produce various cytokines, we examined the relationships between cerebrospinal fluid (CSF) cytokines and TAMs. CSF interleukin‐6 (IL‐6) and soluble IL‐2 receptor were not correlated with the infiltration rate of TAMs; however, CSF IL‐10 level was correlated with infiltration levels of CD68 and CD163+TAMs. We also confirmed the expression of IL‐10 in CD68+and CD163+TAMs by double immunostaining analysis. Our results indicate that a high level of IL‐10 in CSF may be positively associated with the infiltration level of TAMs in PCNSLs.