Clonal Mature Adipocyte Production of Proliferative-competent Daughter Cells Requires Lipid Export Prior to Cell Division.
Clonal Mature Adipocyte Production of Proliferative-competent Daughter Cells Requires Lipid Export Prior to Cell Division.
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DOI：
10.15283/ijsc.2009.2.1.76
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发表时间：
2009-05
影响因子：
2.3
通讯作者：
Jie Chen;M. Guridi;M. Fernyhough;Zhihua Jiang;L. Guan;G. Hausman;M. Dodson
中科院分区：
文献类型：
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作者：
Jie Chen;M. Guridi;M. Fernyhough;Zhihua Jiang;L. Guan;G. Hausman;M. Dodson
背景与目的大量的体外观察表明，成熟的脂肪细胞可以恢复增殖，并开始填充脂肪成纤维细胞部分或形成其他类型的细胞。方法和结果在本研究中，我们评估了成熟猪源性脂肪细胞的克隆培养，因为它们开始重建其分裂能力。细胞质中的脂类要么移动到细胞的顶端，要么从细胞中物理地挤出大液滴。在后一种情况下，我们确定细胞脂滴的处理方式与其他发表的研究中所描述的牛肉脂肪细胞的处理方式不同。结论猪脂肪细胞在细胞分裂前会将大量的脂肪直接排出到培养环境中，而不是像牛肉细胞那样保留所有的脂肪。这种不同的脂肪处理和运输可能是脂肪细胞恢复增殖的新机制。
BACKGROUND AND OBJECTIVES Numerous in vitro observations have been published to show that mature adipocytes may resume proliferation and begin to populate the adipofibroblast fraction or form other cell types. METHODS AND RESULTS In the present study, we evaluated clonal cultures of mature pig-derived adipocytes as they began to reestablish their ability to divide. The lipid contained within the cytoplasm was either moved to the apical ends of the cell, or large droplets were physically extruded from the cell. In the latter case, we ascertained that the cell lipid droplet was handled in a different manner to that by beef-derived adipocytes as described in other published studies. CONCLUSIONS Pig-derived adipocytes expel large amounts of lipid directly into the medium environment prior to becoming capable of cell division, rather than retaining all lipids like the beef cells. This difference in lipid handling and trafficking may be a novel mechanism in adipocyte resumption of proliferation.