Berberine alleviates rotenone-induced cytotoxicity by antioxidation and activation of PI3K/Akt signaling pathway in SH-SY5Y cells
Berberine alleviates rotenone-induced cytotoxicity by antioxidation and activation of PI3K/Akt signaling pathway in SH-SY5Y cells
复制标题
小檗碱通过抗氧化和激活 SH-SY5Y 细胞中 PI3K/Akt 信号通路减轻鱼藤酮诱导的细胞毒性
DOI：
10.1097/wnr.0000000000001365
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发表时间：
2020-01-08
期刊：
影响因子：
1.7
通讯作者：
Ma, ZeGang
中科院分区：
文献类型：
--
作者：
Deng, Han;Jia, Yi;Ma, ZeGang
小檗碱是一种从传统中药中分离得到的异喹啉类生物碱，因其对神经退行性疾病的治疗作用而被广泛研究。然而，小檗碱介导的神经保护在帕金森病的发病机制仍然是不确定的。本实验研究了小檗碱对鱼藤酮诱导的SH-SY 5 Y细胞神经毒性的影响。结果表明，小檗碱处理显著减轻鱼藤酮诱导的SH-SY 5 Y细胞活力下降。进一步研究表明，小檗碱可抑制鱼藤酮诱导的细胞内活性氧的产生，恢复线粒体跨膜电位，增加Bcl-2/Bax比值，降低caspase-3的激活。此外，小檗碱还恢复Akt的磷酸化，这是下调鱼藤酮在SH-SY 5 Y细胞。以上结果提示，小檗碱通过抗氧化和激活PI 3 K/Akt信号通路对鱼藤酮处理的SH-SY 5 Y细胞具有保护作用。NeuroReport 31：41-47版权所有（c）2019威科医疗集团All rights reserved.
Berberine, an isoquinoline alkaloid isolated from traditional Chinese medicine, has been widely studied for its efficacy in the treatment of neurodegenerative diseases. However, berberine-mediated neuroprotection in the pathogenesis of Parkinson's disease is still uncertain. In this study, the effects of berberine on rotenone-induced neurotoxicity in SH-SY5Y cells were investigated. The results showed that berberine treatment significantly alleviated rotenone-induced decrease in the cell viability in SH-SY5Y cells. Further studies demonstrated that berberine suppressed the production of intracellular reactive oxygen species, restored the mitochondrial transmembrane potential, increased Bcl-2/Bax ratio, and decreased caspase-3 activation that induced by rotenone. Furthermore, berberine also restored the phosphorylation of Akt, which was downregulated by rotenone in SH-SY5Y cells. These results suggest that berberine protects rotenone-treated SH-SY5Y cells by antioxidation and activation of PI3K/Akt signaling pathway. NeuroReport 31: 41-47 Copyright (c) 2019 Wolters Kluwer Health, Inc. All rights reserved.