After-effects of repetitive anodal transcranial direct current stimulation on learning and memory in a rat model of Alzheimer's disease
After-effects of repetitive anodal transcranial direct current stimulation on learning and memory in a rat model of Alzheimer's disease
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重复阳极经颅直流电刺激对阿尔茨海默病大鼠模型学习记忆的后遗症
DOI：
10.1016/j.nlm.2019.02.002
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发表时间：
2019
影响因子：
2.7
通讯作者：
Tian Xue Long
中科院分区：
文献类型：
--
作者：
Yang Wen Juan;Wen Hui Zhong;Zhou Lu Xu;Luo Yin Pei;Hou Wen Sheng;Wang Xing;Tian Xue Long
在阿尔茨海默病（AD）大鼠模型中，重复阳极经颅直流电刺激（tDCS）已被证明具有明显的神经保护作用。此外，阳极tDCS的影响不仅发生在刺激期间，而且在刺激结束后持续存在（后效）。在这里，根据我们以前的研究，研究了重复阳极tDCS诱导的后效的持续时间。成年雄性SD大鼠随机分为假手术组（Sham）、β淀粉样蛋白（Aβ）组（AD组）和刺激组（ATD组）。AD组和ATD组大鼠双侧大脑中动脉注射Aβ建立AD模型。ATD组大鼠进行10次阳极tDCS，通过行为学和组织学分析评估重复阳极tDCS的后效。每月使用Morris水迷宫（MWM）评估空间学习和记忆能力。ATD组比AD组表现出更短的逃避潜伏期和更多的平台区域穿越。在最后一次MWM评估后进行海马胆碱乙酰转移酶（ChAT）和胶质细胞酸性蛋白（GFAP）免疫组织化学分析。免疫组化结果显示组间差异显著，尤其是AD组和ATD组之间。这项研究表明，重复的阳极tDCS不仅可以改善认知功能和记忆表现，而且还具有持续2个月的长期后遗症。
Repetitive anodal transcranial direct current stimulation (tDCS) in a rat model of Alzheimer’s disease (AD) has been shown to have distinct neuroprotective effects. Moreover, the effects of anodal tDCS not only occur during the stimulation but also persist after the stimulation has ended (after-effects). Here, the duration of the after-effects induced by repetitive anodal tDCS was investigated based on our previous studies. Adult male Sprague-Dawley rats were divided into three groups: a sham group, a β-amyloid (Aβ) group (AD group) and a stimulation group (ATD group). Aβ was injected into the bilateral hippocampi of the rats in the AD and ATD groups to produce the AD model. Rats in the ATD group underwent 10 sessions of anodal tDCS, and the after-effects of repetitive anodal tDCS were evaluated by behavioral and histological analyses. A Morris water maze (MWM) was utilized on a monthly basis to assess spatial learning and memory abilities. The ATD group showed shorter escape latencies and more platform region crossings than the AD group. Hippocampal choline acetyltransferase (ChAT) and glial fibrillary acidic protein (GFAP) immunohistochemical analyses were carried out after the last MWM assessment. The immunohistochemistry results showed notable differences among the groups, particularly between the AD and ATD groups. This study reveals that repetitive anodal tDCS can not only improve cognitive function and memory performance but also has long-term after-effects that persist for 2 months.