Linkage mapping of AFLP markers in a wild population of great reed warblers:: importance of heterozygosity and number of genotyped individuals
Linkage mapping of AFLP markers in a wild population of great reed warblers:: importance of heterozygosity and number of genotyped individuals
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DOI：
10.1111/j.1365-294x.2007.03290.x
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发表时间：
2007-06-01
影响因子：
4.9
通讯作者：
Bensch, Staffan
中科院分区：
文献类型：
--
作者：
Akesson, Mikael;Hansson, Bengt;Bensch, Staffan
扩增片段长度多态性（AFLP）是显性标记，经常用于建立连锁图谱，杂合性可以推断回交育种策略。在本研究中，我们描述了利用未经处理的大苇莺，Acrocephalus arundinaceus谱系，以推断杂合基因型的AFLP标记，以这些标记的部分连锁图先前的基础上微卫星。总的来说，83个常染色体AFLP位点中的50个（60%）和5个Z连锁AFLP位点中的4个（80%）被定位。对于每个标记，平均检测到杂合子亲本的预期数量的88%。地图分配的可能性在很大程度上是由于微卫星标记的数量和密度已经在地图上。“简约连锁图”，即基于所有显着连锁标记的最简约位置的图，由21个常染色体连锁群组成，具有2至15个标记，总图幅在男性中为552 cM，在女性中为858 cM。由12个标记组成的Z染色体连锁群大小为155 cM。常染色体的“框架连锁图”，这是地图的基础上，只有一个明确的位置标记，有一个总的大小为237 cM的男性和440 cM的女性，分别。AFLP的加入大大扩大了以前的图谱（例如，雄性和雌性重组的常染色体简约连锁图谱分别增加了441 cM和621 cM）。AFLP被映射的概率随着杂合性水平的增加而增加，而映射到框架位置的概率随着杂合性和基因型个体的数量而增加。我们的研究结果表明，AFLP提供了一个快速和廉价的手段扩大遗传图谱已经组成的标记具有高度多态性，也在野生种群与未经处理的系谱。
Amplified fragment length polymorphisms (AFLP) are dominant markers frequently used to build linkage maps where heterozygosity could be inferred by a backcross breeding strategy. In the present study, we describe the utilization of an unmanipulated great reed warbler, Acrocephalus arundinaceus pedigree to infer heterozygous genotypes of AFLP markers in order to map these markers to a partial linkage map previously based on microsatellites. In total, 50 of the 83 autosomal AFLPs (60%) and 4 of 5 Z-linked AFLPs (80%) were mapped. For each marker, on average, 88% of the expected number of heterozygote parents was detected. The likelihood of map assignment was to a large extent due to the number and density of microsatellite markers already in the map. The 'parsimonious linkage map', that is the map based on the most parsimonious location of all significantly linked markers, consisted of 21 autosomal linkage groups with 2 to 15 markers and had a total map size of 552 cM in males and 858 cM in females. The Z-chromosome linkage group with 12 markers had a size of 155 cM. The autosomal 'framework linkage map', that is the map based only on markers with an unambiguous position, had a total size of 237 cM in males and 440 cM in females, respectively. The inclusion of AFLPs enlarged the previous map substantially (e.g. the autosomal parsimonious linkage map became 441 cM and 621 cM larger for male and female recombination, respectively). The probability that an AFLP became mapped increased with increasing level of heterozygosity, whereas the probability of mapping into a framework position increased with both heterozygosity and number of genotyped individuals. Our results suggest that AFLP provides a fast and inexpensive means of enlarging genetic maps already composed of markers with high polymorphism, also in wild populations with unmanipulated pedigrees.