Allelic and haplotype frequencies of the p53 polymorphisms in brain tumor patients
Allelic and haplotype frequencies of the p53 polymorphisms in brain tumor patients
复制标题
DOI：
10.33549/physiolres.930134
复制
发表时间：
2002-01-01
影响因子：
2.1
通讯作者：
Sulla, I
中科院分区：
文献类型：
--
作者：
Biros, E;Kalina, I;Sulla, I
肿瘤抑制基因p53外显子4（p53 BstUI）和内含子6（p53 MspI）的多态性已被认为与多种类型的人类癌症的遗传决定的易感性相关。在我们的医院为基础的病例对照研究中，我们研究了这些多态性的等位基因和基因型的发生率，以及它们的单倍型组合在60例脑肿瘤患者（27名男性和33名女性）和183名对照无恶性肿瘤。从外周血中提取DNA，采用基于PCR的RFLP方法确定基因型特征。在这项研究中，我们发现p53 BstUI和p53 MspI多态性与脑肿瘤风险增加无关。因此，我们得出结论，p53 BstUI和p53 MspI多态性位点内的肿瘤抑制基因p53不代表遗传因素的易感性脑肿瘤。
The polymorphisms of the tumor suppressor gene p53 in exon 4 (p53 BstUI) and in intron 6 (p53 MspI) have been suggested to be associated with the genetically determined susceptibility in diverse types of human cancer. In our hospital-based case-control study, we examined the allele and genotype incidence of these polymorphisms as well as their haplotype combinations in 60 brain tumor patients (27 males and 33 females) and 183 controls without malignancies. The genotype characteristics were determined by the PCR-based RFLP method using DNA extracted from peripheral blood. In this study we show that the p53 BstUI and the p53 MspI polymorphisms are not associated with increased risk of brain tumors. Thus, we conclude that the p53 BstUI and the p53 MspI polymorphic sites within the tumor suppressor gene p53 do not represent genetic determinants of susceptibility to brain tumors.