Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet
Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet
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DOI：
10.1007/s10856-017-5985-5
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发表时间：
2017-09
期刊：
影响因子：
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通讯作者：
Azizah Intan Pangesty;T. Arahira;M. Todo
中科院分区：
文献类型：
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作者：
Azizah Intan Pangesty;T. Arahira;M. Todo
摘要组织工程技术提供了一种替代方法，通过将自体细胞种植到可生物降解的支架中，使血管移植物具有与天然组织相似的生长潜力。在这项研究中，我们开发了一种结合技术，通过将一片细胞分层到多孔管状支架上。人骨髓间充质干细胞(HMSCs)和血管内皮细胞(ECs)共培养制备的细胞片在培养一周内可通过管状聚丙交酯-己内酯(PlcL)支架的多孔结构渗透，并在管腔壁上进一步增殖。此外，在管状支架内共培养的细胞片比仅由MSCs组成的单一培养的细胞片显示出更快的增殖速度。在培养2周内，与单纯培养的细胞片相比，共培养的细胞片表达血管内皮细胞生长因子(VEGF)及其受体(VEGFR)等血管生成标志物，提示共培养体系可以诱导分化为内皮细胞系。这一组合技术将在相对较短的时间内提供血管结构的细胞化和成熟，并强烈表达血管生成特性。
AbstractTissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture. Moreover, the co-culture cell sheet within the tubular scaffold has demonstrated a faster proliferation rate than the monoculture cell sheet composed of MSCs only. We also found that the co-culture cell sheet expressed a strong angiogenic marker, including vascular endothelial growth factor (VEGF) and its receptor (VEGFR), as compared with the monoculture cell sheet within 2 weeks of culture, indicating that the co-culture system could induce differentiation into endothelial cell lineage. This combined technique would provide cellularization and maturation of vascular construct in relatively short period with a strong expression of angiogenic properties.Graphical abstract