MiRNA expression in the cartilage of patients with osteoarthritis.
MiRNA expression in the cartilage of patients with osteoarthritis.
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DOI：
10.1186/s13018-017-0542-y
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发表时间：
2017-03-28
影响因子：
2.6
通讯作者：
Snela S
中科院分区：
文献类型：
--
作者：
Kopańska M;Szala D;Czech J;Gabło N;Gargasz K;Trzeciak M;Zawlik I;Snela S
骨关节炎（OA）是最常见的关节疾病，是一种由遗传、机械和环境因素引起的复杂的多因素疾病。在这项研究中，我们评估了OA中miRNA的表达。对29例接受OA关节置换术的患者的40份组织样本进行了评价。来自两名接受与OA无关的髋关节置换术的对照患者的组织用作对照。使用mirVana miRNA分离试剂盒分离来自软骨的总RNA（含有miRNA种类）。通过实时定量聚合酶链反应评估19种miRNA的表达。在OA组织中，四种miRNA（miR-138- 5 p、miR-146 a-5 p、miR-335- 5 p和miR-9- 5 p）的表达显著上调（患者vs.对照组）。这些发现可能有助于疾病预防和OA治疗靶点的开发。
Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). These findings may contribute to disease prevention and the development of therapeutic targets for OA.