Localization of lysobisphosphatidic acid-rich membrane domains in late endosomes
Localization of lysobisphosphatidic acid-rich membrane domains in late endosomes
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DOI：
10.1515/bc.2001.059
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发表时间：
2001-03-01
影响因子：
3.7
通讯作者：
Gruenberg, J
中科院分区：
文献类型：
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作者：
Kobayashi, T;Startchev, K;Gruenberg, J
晚期内涵体在细胞器腔内积累内膜。这些内膜富含晚期内体特异性磷脂、溶血双磷脂酸 [LBPA]。富含 LBPA 的膜域的组织尚未得到很好的表征。使用 LBPA 特异性单克隆抗体 (6C4)，我们发现这些膜结构域无法从细胞质中进入。使用荧光相关光谱，我们还表明6C4仅结合超声处理的晚期内体，但不结合完整的晚期内体，这可能反映了内体破裂时内膜的释放。
Late endosomes accumulate internal membranes within the lumen of the organelle. These internal membranes are enriched in the late endosome specific phospholipid, lysobisphosphatidic acid [LBPA]. The organization of LBPA-rich membrane domains is not well characterized. Using an LBPA-specific monoclonal antibody (6C4), we show that these membrane domains are not accessible from the cytoplasm. Using fluorescence correlation spectroscopy, we also show that 6C4 only binds sonicated, but not intact, late endosomes, presumably reflecting the release of internal membranes upon endosome rupture.