Characterization of one neutral and two acidic glycoasparagines isolated from the urine of patients with aspartylglycosylaminuria (AGU).
Characterization of one neutral and two acidic glycoasparagines isolated from the urine of patients with aspartylglycosylaminuria (AGU).
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从天冬氨酰糖基胺尿症 (AGU) 患者尿液中分离出的一种中性糖基天冬酰胺和两种酸性糖基天冬酰胺的表征。
DOI：
10.1093/oxfordjournals.jbchem.a131264
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发表时间：
1976
影响因子：
2.7
通讯作者：
I. Yamashina
中科院分区：
文献类型：
--
作者：
K. Sugahara;S. Funakoshi;I. Funakoshi;P. Alla;I. Yamashina
从尿中分离出一种中性和两种酸性糖天冬酰胺。中性的一个被鉴定为β-Gal-（1导致4）-β-GlcNAc-Asn。酸性酶由1摩尔唾液酸和2摩尔半乳糖和N-乙酰葡糖胺组成，连接到天冬酰胺上，并且相对于唾液酸连接的位置是异构的，因为它们在与产气荚膜梭菌的神经氨酸酶[EC 3.2.1.18]孵育时产生相同的糖天冬酰胺。主要基于连续酶促降解的结果，确定所得无唾液酸糖天冬酰胺的结构为β-Gal-β-GlcNAc-β-Gal-（1导致4）-β-GlcNAc-Asn。
One neutral and two acidic glycoasparagines were isolated from the urine of patients with aspartylglycosylaminuria (AGU). The neutral one was identified as beta-Gal-(1 leads to 4)-beta-GlcNAc-Asn. The acidic ones were composed of 1 mole of sialic acid and 2 moles each of galactose and N-acetylglucosamine, attached to asparagine, and were isomeric with respect to the position of sialic acid attachment since they produced the same glycoasparagine on incubation with the neuraminidase [EC 3.2.1.18] from Clostridium perfringens. The structure of the resulting sialic acid-free glycoasparagine was determined to be beta-Gal-beta-GlcNAc-beta-Gal-(1 leads to 4)-beta-GlcNAc-Asn based mainly on the results of sequential enzymatic degradations.