CANDIDA-ALBICANS GENE ENCODING RESISTANCE TO BENOMYL AND METHOTREXATE IS A MULTIDRUG-RESISTANCE GENE
CANDIDA-ALBICANS GENE ENCODING RESISTANCE TO BENOMYL AND METHOTREXATE IS A MULTIDRUG-RESISTANCE GENE
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DOI：
10.1128/aac.38.4.648
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发表时间：
1994-04-01
影响因子：
4.9
通讯作者：
KOLTIN, Y
中科院分区：
文献类型：
--
作者：
BENYAACOV, R;KNOLLER, S;KOLTIN, Y
白色念珠菌不受一些已知影响真菌细胞的药物的抑制。在大多数情况下，这种抗性的基础是未知的，但已归因于真菌细胞包膜的一般不渗透性。一种基因（BEN(r)）以前被证明对白念珠菌对苯甲酰和甲氨蝶呤的耐药性负责，在本研究中被证明对其他四种抑制性化合物：环己亚胺、苯三唑、4-硝基喹啉-n -氧化物和甲基磺美脲具有耐药性。蛋白质数据库分析显示，白色念珠菌基因与原核生物和真核生物中编码抗生素耐药的膜蛋白基因明显相似，与最近克隆的麦芽糖假丝酵母中编码环己亚胺耐药的基因高度同源。我们提出BEN(r)编码一种蛋白质，其运作方式与其他多重耐药蛋白相似，但不完全相同。
Candida albicans is not inhibited by a number of drugs known to affect fungal cells. The basis for this resistance in most cases is unknown but has been attributed to the general impermeability of the fungal cell envelope. A gene (BEN(r)) formerly shown to be responsible for the resistance of C. albicans to benomyl and methotrexate was shown in the present study to confer resistance to four other inhibitory compounds: cycloheximide, benztriazoles, 4-nitroquinoline-N-oxide, and sulfometuron methyl. Analysis of the protein database revealed an apparent similarity of the C. albicans gene to membrane protein genes encoding antibiotic resistance in prokaryotes and eukaryotes and a high degree of identity to a recently cloned gene encoding cycloheximide resistance in Candida maltosa. We propose that BEN(r) encodes a protein that operates in a fashion similar, but not identical, to that described for other multiple-drug resistance proteins.