Combining selected reaction monitoring with discovery proteomics in limited biological samples
Combining selected reaction monitoring with discovery proteomics in limited biological samples
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DOI：
10.1002/pmic.200900310
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发表时间：
2009-11-01
期刊：
影响因子：
3.4
通讯作者：
Kim, Kwang Pyo
中科院分区：
文献类型：
--
作者：
Gupta, Mukesh Kumar;Jung, Jin Woo;Kim, Kwang Pyo
通过选择性反应监测（SRM）同时定量多种蛋白质在细胞信号研究中有许多应用，包括胚胎蛋白质组学。然而，由于所选肽与多个非相关蛋白可能存在离子冗余和/或序列相似性，最近人们对SRM检测的特异性提出了担忧。在这篇观点文章中，我们讨论了一些简单的措施，可以提高我们对SRM扫描在蛋白质组学实验中的准确性的信心。至少在来自猪种的胚胎样本中，这些方法被发现在验证ms鉴定的差异表达蛋白方面是有用的。在SRM分析的9个蛋白中，MS实验中发现的上调或下调的蛋白在SRM实验中也忠实地上调或下调。
Simultaneous quantification of multiple proteins by selected reaction monitoring (SRM) has several applications in cell signaling studies including embryo proteomics. However, concerns have recently been raised over the specificity of SRM assays due to possible ion redundancy and/or sequence similarity of selected peptide with multiple non-related proteins. In this Viewpoint article, we discuss some simple measures that can increase our confidence in the accuracy of SRM scans used in proteomic experiments. At least in embryonic samples from porcine species, these measures were found to be useful in validating MS-identified differentially expressed proteins. Among the nine proteins analyzed by SRM assay, all the proteins that were found to be up- or down-regulated in MS experiment were also faithfully up- or down-regulated in SRM assay.