ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration.
ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration.
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DOI：
10.2147/ijn.s269169
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发表时间：
2020
影响因子：
8
通讯作者：
Chen V
中科院分区：
文献类型：
--
作者：
Ejeian F;Razmjou A;Nasr-Esfahani MH;Mohammad M;Karamali F;Ebrahimi Warkiani M;Asadnia M;Chen V
尽管在细胞微环境的生物力学方面的建模取得了重大进展，但精确模拟特定细胞生态位的生理条件仍然是一个重大挑战。在这里，金属有机框架（MOFs）已被引入作为一个可行的平台，多因素控制细胞-基底相互作用，考虑到广泛的物理和机械性能的MOF材料及其结构的灵活性。原位结晶的沸石咪唑骨架-8（ZIF-8）上的聚多巴胺（PDA）改性的膜显着提高了表面能，润湿性，粗糙度和刚度的基板。与传统的塑料培养皿相比，这种调制导致牙髓干细胞（DPSC）的初级附着几乎增加了两倍。研究结果表明，PDA/ZIF-8涂层改性的聚丙烯（PP）膜有效地支持DPSCs的生长和增殖，在相当大的速度。进一步的分析还显示了DPSC的夸大的多谱系分化，具有放大水平的自分泌细胞命运决定信号，如BSP 1、BMP 2、PPARG、FABP 4、ACAN和COL 2A。值得注意的是，成骨标志物响应于ZIF-8层的生物力学特性而显著过表达（超过100倍，而不是组织培养板）。因此，用MOF纳米结构对细胞培养平台进行表面修饰被提出作为用于选择性地引导干细胞用于组织再生的强大的纳米医学方法。PP/PDA/ZIF-8复合膜是一种理想的牙周组织工程引导性骨再生屏障膜。
Despite the significant advances in modeling of biomechanical aspects of cell microenvironment, it remains a major challenge to precisely mimic the physiological condition of the particular cell niche. Here, the metal–organic frameworks (MOFs) have been introduced as a feasible platform for multifactorial control of cell-substrate interaction, given the wide range of physical and mechanical properties of MOF materials and their structural flexibility. In situ crystallization of zeolitic imidazolate framework-8 (ZIF-8) on the polydopamine (PDA)-modified membrane significantly raised surface energy, wettability, roughness, and stiffness of the substrate. This modulation led to an almost twofold increment in the primary attachment of dental pulp stem cells (DPSCs) compare to conventional plastic culture dishes. The findings indicate that polypropylene (PP) membrane modified by PDA/ZIF-8 coating effectively supports the growth and proliferation of DPSCs at a substantial rate. Further analysis also displayed the exaggerated multilineage differentiation of DPSCs with amplified level of autocrine cell fate determination signals, like BSP1, BMP2, PPARG, FABP4, ACAN, and COL2A. Notably, osteogenic markers were dramatically overexpressed (more than 100-folds rather than tissue culture plate) in response to biomechanical characteristics of the ZIF-8 layer. Hence, surface modification of cell culture platforms with MOF nanostructures proposed as a powerful nanomedical approach for selectively guiding stem cells for tissue regeneration. In particular, PP/PDA/ZIF-8 membrane presented ideal characteristics for using as a barrier membrane for guided bone regeneration (GBR) in periodontal tissue engineering.