The ERK-1 function is required for HSV-1-mediated G1/S progression in HEP-2 cells and contributes to virus growth.
The ERK-1 function is required for HSV-1-mediated G1/S progression in HEP-2 cells and contributes to virus growth.
复制标题
HSV-1介导的HEP-2细胞中HSV-1介导的G1/S进展需要ERK-1功能，并有助于病毒生长。
DOI：
10.1038/s41598-017-09529-y
复制
发表时间：
2017-08-23
影响因子：
4.6
通讯作者：
Sciortino MT
中科院分区：
文献类型：
--
作者：
Colao I;Pennisi R;Venuti A;Nygårdas M;Heikkilä O;Hukkanen V;Sciortino MT
The herpes simplex virus 1 is able to readdress different cellular pathways including cell cycle to facilitate its replication and spread. During infection, the progression of the cell cycle from G1 to S phase makes the cellular replication machinery accessible to viral DNA replication. In this work we established that HSV-1, in asynchronized HEp-2 cells, strictly controls cell cycle progression increasing S-phase population from 9 hours post infection until the end of HSV-1 replication. The G1/S phases progression depends on two important proteins, cyclin E and CDK2. We demonstrate that their phosphorylated status and then their activity during the infection is strongly correlated to viral replication events. In addition, HSV-1 is able to recruit and distribute ERK1/2 proteins in a spatio-temporal fashion, highlighting its downstream regulatory effects on cellular processes. According with this data, using chemical inhibitor U0126 and ERK dominant negative cells we found that the lack of ERK1 activity affects cyclin E protein accumulation, viral gene transcription and percentage of the cells in S phase, during the viral replication. These data suggested a complex interaction between ERK, cell cycle progression and HSV-1 replication.
登录
查看更多内容
影响因子：
3.8
作者：
Hossain, A;Holt, T;Jones, C
通讯作者：
Jones, C
DOI：
10.1073/pnas.0906905106
发表时间：
2009-08-25
影响因子：
11.1
作者：
Kalamvoki, Maria;Roizman, Bernard
通讯作者：
Roizman, Bernard
影响因子：
4.8
作者：
Lents, NH;Keenan, SM;Baldassare, JJ
通讯作者：
Baldassare, JJ
影响因子：
3.7
作者：
Ehmann, GL;McLean, TI;Bachenheimer, SL
通讯作者：
Bachenheimer, SL
影响因子：
4.8
作者：
Favata, MF;Horiuchi, KY;Trzaskos, JM
通讯作者：
Trzaskos, JM