TANC1 methylation as a novel biomarker for the diagnosis of patients with anti-tuberculosis drug-induced liver injury.
TANC1 methylation as a novel biomarker for the diagnosis of patients with anti-tuberculosis drug-induced liver injury.
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TANC1甲基化作为诊断抗结核药物性肝损伤患者的新型生物标志物
DOI：
10.1038/s41598-021-96869-5
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发表时间：
2021-08-31
影响因子：
4.6
通讯作者：
Feng F
中科院分区：
文献类型：
--
作者：
Wu D;Li Y;Ren Q;Pei S;Wang L;Yang L;Chong Y;Sun S;Hao J;Feng F
我们旨在阐明ADLI和非ADLI患者之间基因组甲基化模式的差异，以确定基于DNA甲基化的生物标志物。采用Infinium MethylationEPIC（EPIC）BeadChip芯片检测14份外周血标本（7例ADLI患者，7例非ADLI对照）的全基因组DNA甲基化模式。另外120例患者（ADLI组60例，非ADLI组60例）外周血标本分别采用实时荧光定量聚合酶链反应（PCR）和焦磷酸测序（Pyrosequencing）方法检测靶基因mRNA和DNA甲基化水平的变化。共鉴定出308个高甲基化CpG位点和498个低甲基化CpG位点。值得注意的是，通过全基因组DNA甲基化分析确定了与ADLI相关的TANC1中高甲基化CpG位点cg06961147和cg24666046。与对照组相比，TANC1的mRNA表达在病例中较低。焦磷酸测序验证了这两个差异甲基化位点，这与EPIC BeadChip阵列的结果一致。受试者操作特征分析表明，TANC1（cg06961147、cg24666046及其组合）的曲线下面积分别为0.812、0.842和0.857。这些结果表明，ADLI患者的基因组甲基化模式与无ADLI患者不同。TANC 1中高甲基化的差异甲基化位点cg06961147与cg24666046组合为ADLI的诊断提供了证据。
We aimed to elucidate the differences in genomic methylation patterns between ADLI and non-ADLI patients to identify DNA methylation-based biomarkers. Genome-wide DNA methylation patterns were obtained using Infinium MethylationEPIC (EPIC) BeadChip array to analyze 14 peripheral blood samples (7 ADLI cases, 7 non-ADLI controls). Changes in the mRNA and DNA methylation in the target genes of another 120 peripheral blood samples (60 ADLI cases, 60 non-ADLI controls) were analyzed by real-time polymerase chain reaction and pyrosequencing, respectively. A total of 308 hypermethylated CpG sites and 498 hypomethylated CpG sites were identified. Significantly, hypermethylated CpG sites cg06961147 and cg24666046 in TANC1 associated with ADLI was identified by genome-wide DNA methylation profiling. The mRNA expression of TANC1 was lower in the cases compared to the controls. Pyrosequencing validated these two differentially methylated loci, which was consistent with the results from the EPIC BeadChip array. Receiver operating characteristic analysis indicated that the area under the curve of TANC1 (cg06961147, cg24666046, and their combinations) was 0.812, 0.842, and 0.857, respectively. These results indicate that patients with ADLI have different genomic methylation patterns than patients without ADLI. The hypermethylated differentially methylated site cg06961147 combined with cg24666046 in TANC1 provides evidence for the diagnosis of ADLI.
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影响因子：
--
作者：
Wessel K;Suleiman J;Khalaf TE;Kishore S;Rolfs A;El-Hattab AW
通讯作者：
El-Hattab AW
DOI：
10.1016/j.jtho.2016.05.010
发表时间：
2016-09
期刊：
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
影响因子：
--
作者：
Wang YW;Ma X;Zhang YA;Wang MJ;Yatabe Y;Lam S;Girard L;Chen JY;Gazdar AF
通讯作者：
Gazdar AF
影响因子：
3.8
作者：
Piras, Ignazio S.;Mills, Gabrielle;Schrauwen, Isabelle
通讯作者：
Schrauwen, Isabelle
影响因子：
5.8
作者：
Aryee, Martin J.;Jaffe, Andrew E.;Irizarry, Rafael A.
通讯作者：
Irizarry, Rafael A.
药物诱导的抗结核药物及其血清水平的肝毒性。
影响因子：
4.5
作者：
Jeong I;Park JS;Cho YJ;Yoon HI;Song J;Lee CT;Lee JH
通讯作者：
Lee JH