Immunostimulatory effects of a plasmid expressing CD40 ligand (CD154) on gene immunization.
Immunostimulatory effects of a plasmid expressing CD40 ligand (CD154) on gene immunization.
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DOI：
10.4049/jimmunol.159.12.5777
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发表时间：
1997-12
影响因子：
4.4
通讯作者：
Robert B. Mendoza;M. Cantwell;T. Kipps
中科院分区：
文献类型：
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作者：
Robert B. Mendoza;M. Cantwell;T. Kipps
CD40与其配体（CD154）的相互作用可以诱导携带CD40的apc表达促进免疫识别的免疫刺激附属分子。我们评估了编码CD154 （pCD40L）的质粒载体是否会影响对共注射质粒DNA编码的转基因蛋白的免疫反应。我们发现，在BALB/c小鼠中共注射pCD40L可增强Ab对β -半乳糖苷酶的反应，这种反应是通过皮下注射编码β -半乳糖苷酶的质粒DNA载体placZ诱导的。此外，皮下注射pCD40L和placZ可增强转染placZ的P815细胞特异性CTL的产生。本研究表明，pCD40L可以作为一种遗传佐剂，增强对质粒DNA表达载体编码的Ags的体液和细胞免疫应答。
Interaction of CD40 with its ligand (CD154) can induce CD40-bearing APCs to express immune stimulatory accessory molecules that facilitate immune recognition. We evaluated whether a plasmid vector encoding CD154 (pCD40L) could influence the immune response to a transgene protein encoded by coinjected plasmid DNA. We found that coinjection of pCD40L in BALB/c mice enhanced the Ab response to beta-galactosidase induced by i.m. or intradermal injection of placZ, a plasmid DNA vector encoding beta-galactosidase. Furthermore, i.m. or intradermal coinjection of pCD40L with placZ enhanced the generation of CTL specific for P815 cells transfected with placZ. This study indicates that pCD40L can serve as a genetic adjuvant capable of augmenting humoral and cellular immune responses to Ags encoded by plasmid DNA expression vectors.