Effect of the extracts from Glycyrrhiza uralensis Fisch on the growth characteristics of human cell lines:: Anti-tumor and immune activation activities
Effect of the extracts from Glycyrrhiza uralensis Fisch on the growth characteristics of human cell lines:: Anti-tumor and immune activation activities
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DOI：
10.1023/a:1016111713056
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发表时间：
2001-01-01
期刊：
影响因子：
2.2
通讯作者：
Lee, HY
中科院分区：
文献类型：
--
作者：
Chung, WT;Lee, SH;Lee, HY
通过保存几种人细胞系的生长特性，研究了甘草菲施醇提物的免疫调节活性。所有样品对正常人肝细胞系WRL-68均未显示出严重的细胞毒性，显示出小于25%的细胞生长抑制。粗提物及其分级样品（F1和F3）抑制人肝癌Hep 3B的生长，抑制率降至约100%。70%的正常细胞生长中加入1.0克升（-1）的F3级分。抗肿瘤实验结果与中国人肺癌细胞株（CHLV 79）的抗突变实验结果吻合良好。在加入1.0 g l（-1）的F1组分时，人B细胞的生长得到增强，达到对照生长的60%。在加入粗提物时，人B细胞的两种细胞因子白细胞介素-6和肿瘤坏死因子-α的分泌也增加，但标准品如甘草甜素（GL）或18，β-甘草次酸（GM）没有增加。结果发现，添加粗提物和F1组分比添加标准品更能促进细胞凋亡和分化。通过薄层色谱法（TLC）分析，仅在粗提物和级分中发现斑点，而不是标准品。这说明F1粗品和/或馏分中一定存在另一种未知成分，可能是免疫调节剂的候选成分。该组分似乎是单体GM的衍生物，因为其Rf接近单体。另外，G. uralensis菲施通过首先用酶水解而被生物活化。
Immune modulating activity of ethanol extracts from Glycyrrhiza uralensis Fisch was investigated by conserving growth characteristics of several human cell lines. All of the samples did not show severe cytotoxicity on normal human liver cell line, WRL-68, showing less than 25% inhibition of cell growth. The crude extract and its fractionized samples (F1 and F3) inhibited the growth of human hepatoma, Hep3B, down to ca. 70% of normal cell growth in adding 1.0 g l(-1) of fraction F3. The result of anticancer experiments was well matched to the results of antimutagenicity using Chinese Hamster Lung cell lines (CHL V79). In adding 1.0 g l(-1) of fraction F1, the growth of human B cell was enhanced, up to 60% of control growth. The secretion of two kinds of cytokines, Interleukin-6 and Tumor Necrosis Factor-alpha from human B cells was also enhanced in adding the crude extract, but not the standards such as Glycyrrhizin (GL) or 18,beta-glycyrrhetinic acid (GM). It was found that both of the apoptosis and differentiation were more accelerated in supplementing the crude extract and fraction F1 than in adding the standards. A spot was found only in the crude extract and fractions, not standards by Thin Layer Chromatography (TLC) analysis. It tells that there must be another unknown component in crude and/or fraction F1 as a possible candidate of immune modulators. This component seems to be a derivative of a monomer, GM since its R-f was close to the monomer. It was also interesting that glycyrrhizin, a major component in G. uralensis Fisch was biologically activated by first being hydrolyzed by an enzyme.