A portable low-cost long-term live-cell imaging platform for biomedical research and education
A portable low-cost long-term live-cell imaging platform for biomedical research and education
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DOI：
10.1016/j.bios.2014.09.061
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发表时间：
2015-02-15
影响因子：
12.6
通讯作者：
Gilbert, Daniel F.
中科院分区：
文献类型：
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作者：
Walzik, Maria P.;Vollmar, Verena;Gilbert, Daniel F.
活细胞慢动态过程的时间分辨可视化和分析已经彻底改变了体外细胞研究的许多方面。然而，应用于时间分辨活细胞显微镜的现有技术通常是固定的，昂贵的，并且需要高水平的技能来使用和维护。这些因素限制了其应用于实地研究和教育目的。最近快速原型技术的可用性使得可以快速轻松地设计出低成本和用户友好的传统研究基础设施的专用替代方案。在本文中，我们描述了一个全自动低成本，便携式活细胞成像系统的原型，用于活细胞动态过程的时间分辨无标签可视化。该设备重量轻（3.6 kg），体积小（22 x 22 x 22 cm），成本极低（< (sic)1250）。我们通过在不同培养条件下对重组HEK293细胞进行长期成像，证明了其在生物医学上的应用潜力，并验证了其产生高质量时间分辨数据的能力，从而可以分析活细胞中的时间依赖性过程。虽然这项工作的重点是哺乳动物细胞的长期成像，但所提出的技术也可以适用于其他生物标本，并为生命科学和教育中应用的快速原型化低成本生物传感器技术提供了一个一般例子。(C) 2014年作者。Elsevier B.V.出版
Time-resolved visualization and analysis of slow dynamic processes in living cells has revolutionized many aspects of in vitro cellular studies. However, existing technology applied to time-resolved live-cell microscopy is often immobile, costly and requires a high level of skill to use and maintain. These factors limit its utility to field research and educational purposes. The recent availability of rapid prototyping technology makes it possible to quickly and easily engineer purpose-built alternatives to conventional research infrastructure which are low-cost and user-friendly. In this paper we describe the prototype of a fully automated low-cost, portable live-cell imaging system for time-resolved label-free visualization of dynamic processes in living cells. The device is light-weight (3.6 kg), small (22 x 22 x 22 cm) and extremely low-cost (< (sic)1250). We demonstrate its potential for biomedical use by long-term imaging of recombinant HEK293 cells at varying culture conditions and validate its ability to generate time-resolved data of high quality allowing for analysis of time-dependent processes in living cells. While this work focusses on long-term imaging of mammalian cells, the presented technology could also be adapted for use with other biological specimen and provides a general example of rapidly prototyped low-cost biosensor technology for application in life sciences and education. (C) 2014 The Authors. Published by Elsevier B.V.