TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization.
TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization.
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DOI：
10.1093/bioinformatics/btt276
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发表时间：
2013-07-15
期刊：
影响因子：
--
通讯作者：
Boudier T
中科院分区：
文献类型：
--
作者：
Ollion J;Cochennec J;Loll F;Escudé C;Boudier T
动机：细胞核是一个高度组织化的细胞器，包含遗传物质。细胞核结构的研究已成为细胞生物学的一个重要领域。从3D荧光成像中提取定量数据有助于了解不同核隔室的功能。然而，这种方法受到处理和分析大图像集的要求的限制。结果如下：在这里，我们描述了核基因组组织分析工具（TANGO），一个致力于研究核结构的图像分析工具。TANGO是一个连贯的框架，允许生物学家通过结合两个环境来执行3D荧光图像的完整分析过程：ImageJ（http：//imagej.nih.gov/ij/）用于图像处理和定量分析，R（http：cran.r-project.org）用于测量结果的统计处理。它包括一个直观的用户界面，提供了精确构建分割程序和设置分析的方法，而无需具备编程技能。TANGO是一个多功能的工具，能够处理大量的图像，允许定量研究核组织。可用性：TANGO由两个程序组成：（i）ImageJ插件和（ii）R软件包（rtango）。它们都是免费和开源的，可用于Linux、Microsoft Windows和Macintosh OSX（http：//biophysique.mnhn.fr/tango）。发布遵循GPL v.2许可证。联系方式：托马斯. snv.jussieu.fr补充信息：补充数据可在生物信息学在线获得。
Motivation: The cell nucleus is a highly organized cellular organelle that contains the genetic material. The study of nuclear architecture has become an important field of cellular biology. Extracting quantitative data from 3D fluorescence imaging helps understand the functions of different nuclear compartments. However, such approaches are limited by the requirement for processing and analyzing large sets of images. Results: Here, we describe Tools for Analysis of Nuclear Genome Organization (TANGO), an image analysis tool dedicated to the study of nuclear architecture. TANGO is a coherent framework allowing biologists to perform the complete analysis process of 3D fluorescence images by combining two environments: ImageJ (http://imagej.nih.gov/ij/) for image processing and quantitative analysis and R (http://cran.r-project.org) for statistical processing of measurement results. It includes an intuitive user interface providing the means to precisely build a segmentation procedure and set-up analyses, without possessing programming skills. TANGO is a versatile tool able to process large sets of images, allowing quantitative study of nuclear organization. Availability: TANGO is composed of two programs: (i) an ImageJ plug-in and (ii) a package (rtango) for R. They are both free and open source, available (http://biophysique.mnhn.fr/tango) for Linux, Microsoft Windows and Macintosh OSX. Distribution is under the GPL v.2 licence. Contact: thomas.boudier@snv.jussieu.fr Supplementary information: Supplementary data are available at Bioinformatics online.
影响因子：
5.8
作者：
Kamentsky, Lee;Jones, Thouis R.;Carpenter, Anne E.
通讯作者：
Carpenter, Anne E.
影响因子：
5.8
作者：
Shirley, James W.;Ty, Sereyvathana;Gilbert, David M.
通讯作者：
Gilbert, David M.
影响因子：
5.8
作者：
Iannuccelli, E.;Mompart, F.;Boudier, T.
通讯作者：
Boudier, T.