Differential expression and regulation of Tdo2 during mouse decidualization
Differential expression and regulation of Tdo2 during mouse decidualization
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小鼠蜕膜化过程中Tdo2的差异表达和调控
DOI：
10.1530/joe-13-0429
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发表时间：
2014-01-01
影响因子：
4
通讯作者：
Yue, Zhan-Peng
中科院分区：
文献类型：
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作者：
Li, Dang-Dang;Gao, Ying-Jie;Yue, Zhan-Peng
色氨酸2,3-双加氧酶（Tdo2）是一种限速酶，指导色氨酸转化为犬尿氨酸。本研究的目的是研究Tdo2在小鼠子宫脱个位化过程中的表达和调控。Tdo2 mRNA主要在妊娠6 ~ 8天蜕膜中表达。通过实时荧光定量PCR检测，妊娠第6 ~ 8天子宫内Tdo2高表达，妊娠第1 ~ 8天子宫内Tdo2高表达。同时，在体内和体外人工脱个别化下检测Tdo2 mRNA。雌激素、黄体酮和8-溴腺苷- camp均可诱导Tdo2在去卵巢小鼠子宫和子宫间质细胞中的表达。Tdo2可调节细胞增殖，刺激子宫间质细胞和蜕膜细胞中蜕膜标志物Dtprp的表达。过表达Tdo2可上调子宫间质细胞中Ahr、Cox2和Vegf基因的表达，而Tdo2抑制剂680C91可下调子宫蜕膜细胞中Cox2和Vegf基因的表达。这些数据表明，Tdo2可能在小鼠去个体化过程中发挥重要作用，并受雌激素、黄体酮和cAMP的调节。
Tryptophan 2,3-dioxygenase (Tdo2) is a rate-limiting enzyme which directs the conversion of tryptophan to kynurenine. The aim of this study was to examine the expression and regulation of Tdo2 in mouse uterus during decidualization. Tdo2 mRNA was mainly expressed in the decidua on days 6-8 of pregnancy. By real-time PCR, a high level of Tdo2 expression was observed in the uteri from days 6 to 8 of pregnancy, although Tdo2 expression was observed on days 1-8. Simultaneously, Tdo2 mRNA was also detected under in vivo and in vitro artificial decidualization. Estrogen, progesterone, and 8-bromoadenosine-cAMP could induce the expression of Tdo2 in the ovariectomized mouse uterus and uterine stromal cells. Tdo2 could regulate cell proliferation and stimulate the expression of decidual marker Dtprp in the uterine stromal cells and decidual cells. Overexpression of Tdo2 could upregulate the expression of Ahr, Cox2, and Vegf genes in uterine stromal cells, while Tdo2 inhibitor 680C91 could downregulate the expression of Cox2 and Vegf genes in uterine decidual cells. These data indicate that Tdo2 may play an important role during mouse decidualization and be regulated by estrogen, progesterone, and cAMP.