Role of Staphylococcus aureus protein A in adherence to silastic catheters.
Role of Staphylococcus aureus protein A in adherence to silastic catheters.
复制标题
金黄色葡萄球菌蛋白 A 在粘附硅橡胶导管中的作用。
DOI：
10.1016/j.jss.2010.12.039
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Hess,DonavonJ
中科院分区：
文献类型：
--
作者：
Henry-Stanley,MichelleJ;Shepherd,MelodyM;Wells,CarolL;Hess,DonavonJ
背景：中心静脉导管上常出现细菌群落，称为生物膜，中心静脉导管的细菌污染是引起医院内血流感染的最常见原因。细菌黏附导管表面的初始事件知之甚少，实验旨在阐明金黄色葡萄球菌与硅胶导管黏附过程中金黄色葡萄球菌蛋白A、血清和免疫球蛋白的作用。我们假设金黄色葡萄球菌蛋白A在血清和免疫球蛋白存在的情况下会改变金黄色葡萄球菌在硅胶导管上的黏附。材料和方法将不同表达金黄色葡萄球菌A蛋白的材料和方法在室温下与硅胶导管培养15min，并对细菌在导管表面的黏附进行定量。结果血清和白蛋白对金黄色葡萄球菌在导管表面的黏附均有抑制作用，而蛋白A的表达对这种作用没有明显的调节作用。纯化的血清免疫球蛋白对金黄色葡萄球菌的黏附也有抑制作用，与高表达A蛋白的金黄色葡萄球菌相比，免疫球蛋白对A蛋白缺乏的金黄色葡萄球菌黏附的抑制作用更强。结论全血清白蛋白和纯化的免疫球蛋白均能抑制金黄色葡萄球菌与硅胶导管的黏附。葡萄球菌蛋白A的表达干扰了Ig G介导的细菌与导管表面结合的抑制作用。蛋白A在免疫球蛋白存在的情况下改变金黄色葡萄球菌对硅胶导管的粘附性，但在血清或白蛋白存在的情况下不改变。
BACKGROUNDCommunities of bacteria, termed biofilms, frequently develop on central venous catheters, and bacterial contamination of central venous catheters is the most common cause of nosocomial bloodstream infections. Little is known about the initial events in bacterial adherence to the catheter surface, and experiments were designed to clarify the role of staphylococcal protein A, serum, and immunoglobulin in adherence of Staphylococcus aureus to silastic catheters. We hypothesized that S. aureus protein A in the presence of serum and immunoglobulin would alter S. aureus adherence to silastic catheters.MATERIALS AND METHODSThree strains of S. aureus with varying expression of staphylococcal protein A were incubated 15 min at room temperature with silastic catheters, and bacterial adherence to the catheter surface was quantified. In addition, the effects of serum, albumin, and purified IgG on bacterial adherence were assessed.RESULTSBoth serum and albumin had an inhibitory effect on S. aureus adherence to the catheter surface, and protein A expression did not appreciably modulate these effects. Purified serum IgG also inhibited S. aureus adherence, with IgG having a greater inhibitory effect on the adherence of an S. aureus strain deficient in protein A compared with an S. aureus strain expressing high levels of protein A.CONCLUSIONS. aureus adherence to silastic catheters was inhibited by whole serum, albumin, and purified IgG. Expression of staphylococcal protein A interfered with IgG mediated inhibition of bacterial binding to the catheter surface. Protein A altered S. aureus adherence to silastic catheters in the presence of immunoglobulin, but not in the presence of serum or albumin.