Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays
Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays
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DOI：
10.1073/pnas.0914218107
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发表时间：
2010-04-06
影响因子：
11.1
通讯作者：
Addadi, Lia
中科院分区：
文献类型：
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作者：
Mahamid, Julia;Aichmayer, Barbara;Addadi, Lia
斑马鱼不断形成的鳍骨射线代表了一个简单的骨骼模型系统，其中矿化在时间和空间上得到了解决。鳍骨的矿化胶原纤维在结构上与所有已知骨材料中发现的结构相同。我们通过使用同步加速器微束 X 射线衍射和小角散射，并结合冷冻扫描电子显微镜来研究组织内的连续矿化过程。前者提供有关矿物相以及矿物颗粒尺寸和形状的信息，而后者可以对天然水合组织进行高分辨率成像。两种技术的整合表明，新的矿物质以无定形磷酸钙纳米球的形式输送和沉积，这些纳米球在胶原蛋白基质内转化为结晶磷灰石的血小板。
The continuously forming fin bony rays of zebrafish represent a simple bone model system in which mineralization is temporally and spatially resolved. The mineralized collagen fibrils of the fin bones are identical in structure to those found in all known bone materials. We study the continuous mineralization process within the tissue by using synchrotron microbeam x-ray diffraction and small-angle scattering, combined with cryo-scanning electron microscopy. The former provides information on the mineral phase and the mineral particles size and shape, whereas the latter allows high-resolution imaging of native hydrated tissues. The integration of the two techniques demonstrates that new mineral is delivered and deposited as packages of amorphous calcium phosphate nanospheres, which transform into platelets of crystalline apatite within the collagen matrix.