Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain.
Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain.
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DOI：
10.1038/ncomms11100
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发表时间：
2016-03-22
影响因子：
16.6
通讯作者：
Hirase H
中科院分区：
文献类型：
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作者：
Monai H;Ohkura M;Tanaka M;Oe Y;Konno A;Hirai H;Mikoshiba K;Itohara S;Nakai J;Iwai Y;Hirase H
经颅直流电刺激（tDCS）是一种已知的治疗方法，可以改善各种神经系统疾病，增强人类的记忆和认知。tDCS因其潜在的治疗价值而受到关注；然而，人们对其作用机制知之甚少。利用在星形胶质细胞和兴奋性神经元亚群中表达G-CaMP7的转基因小鼠，我们发现tDCS诱导星形胶质细胞Ca2+在整个皮质中大幅增加，而局部场电位没有明显变化。此外，tDCS后感觉诱发的皮层反应增强。这些增强依赖于α -1肾上腺素能受体，在IP3R2（肌醇三磷酸受体2型）敲除小鼠中未观察到，其中星形细胞Ca2+激增不存在。总之，我们提出tDCS通过星形细胞Ca2+/IP3信号改变皮层的元可塑性。虽然经颅直流电刺激（tDCS）已被应用于临床，但其细胞作用机制尚不清楚。在这里，Hajime Hirase和他的同事观察了小鼠大脑对tDCS的细胞反应，并显示了与可塑性变化相一致的强大的星形胶质细胞激活。
Transcranical direct current stimulation (tDCS) is a treatment known to ameliorate various neurological conditions and enhance memory and cognition in humans. tDCS has gained traction for its potential therapeutic value; however, little is known about its mechanism of action. Using a transgenic mouse expressing G-CaMP7 in astrocytes and a subpopulation of excitatory neurons, we find that tDCS induces large-amplitude astrocytic Ca2+ surges across the entire cortex with no obvious changes in the local field potential. Moreover, sensory evoked cortical responses are enhanced after tDCS. These enhancements are dependent on the alpha-1 adrenergic receptor and are not observed in IP3R2 (inositol trisphosphate receptor type 2) knockout mice, in which astrocytic Ca2+ surges are absent. Together, we propose that tDCS changes the metaplasticity of the cortex through astrocytic Ca2+/IP3 signalling. While transcranical direct current stimulation (tDCS) is used in clinical setting, its cellular mechanism of action is unclear. Here, Hajime Hirase and colleagues visualize cellular response in mouse brain to tDCS and show robust astrocyte activation that coincide with plasticity changes.