DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI
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DOI：
10.1016/s1097-2765(00)80146-x
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发表时间：
1998-10-01
期刊：
影响因子：
16
通讯作者：
Stoddard, BL
中科院分区：
文献类型：
--
作者：
Jurica, NS;Monnat, RJ;Stoddard, BL
与归巢位点 DNA 结合的 LAGLIDADG 内含子编码的归巢核酸内切酶 I-Crel 的结构已确定。该界面由每个酶单体延伸的凹形β片层形成，接触每个DNA半位点，从而导致侧链与全长归巢位点的24个碱基对中的18个直接接触。该结构表明，I-Crel 通过表现出长位点识别能力，同时能够切割许多密切相关的靶序列，对其在遗传转座中的作用进行了优化。 DNA 切割是由 I-Crel 同二聚体中一对紧凑的活性位点介导的，每个活性位点都包含一个单独的结合二价阳离子。
The structure of the LAGLIDADG intron-encoded homing endonuclease I-Crel bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-Crel is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-Crel homodimer, each of which contains a separate bound divalent cation.