Inhibitory effect of mulberroside A and its derivatives on melanogenesis induced by ultraviolet B irradiation
Inhibitory effect of mulberroside A and its derivatives on melanogenesis induced by ultraviolet B irradiation
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DOI：
10.1016/j.fct.2011.09.008
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发表时间：
2011-12-01
影响因子：
4.3
通讯作者：
Lim, Young-Hee
中科院分区：
文献类型：
--
作者：
Park, Keun-Tae;Kim, Jeong-Keun;Lim, Young-Hee
桑黄皂苷A是从蒙古根乙醇提取物中分离得到的。桑黄苷A经Pectinex(R)酶解后生成氧化白藜芦醇和氧化白藜芦醇-3-O-葡萄糖苷。我们测试了氧化白藜芦醇、氧化白藜芦醇-3-O-葡萄糖苷和桑黄皂苷A，以确定它们是否能抑制中波紫外线(UVB)辐射诱导的棕色豚鼠皮肤黑素生成。局部应用桑黄苷A、氧化白藜芦醇和氧化白藜芦醇-3-O-葡萄糖苷可减少豚鼠皮肤的色素沉着。这些化合物抑制黑色素生成酶酪氨酸酶、酪氨酸酶相关蛋白-1和小眼球转录因子的表达。其中，氧化白藜芦醇的抗黑素作用最强，其次是氧化白藜芦醇-3-O-葡萄糖苷，桑皂苷A的抗黑素作用最差。桑黄皂苷A是氧化白藜芦醇的糖基化二苯乙烯类化合物，因此，桑白藜芦醇A的脱糖基化对黑素合成的抑制作用增强。Fontana-Masson染色的组织学分析证实，与赋形剂对照组相比，这些化合物显著降低了UVB照射的豚鼠皮肤表皮中的黑色素含量。因此，这些化合物有效地减少了色素沉着，可能是适合皮肤美白的美容剂。(C)2011爱思唯尔有限公司。保留所有权利。
Mulberroside A was isolated from the ethanol extract of Mons alba roots. The enzymatic hydrolysis of mulberroside A with Pectinex (R) produced oxyresveratrol and oxyresveratrol-3-O-glucoside. We tested oxyresveratrol, oxyresveratrol-3-O-glucoside, and mulberroside A to determine whether they could inhibit ultraviolet B (UVB) irradiation-induced melanogenesis in brown guinea pig skin. Topical application of mulberroside A, oxyresveratrol, and oxyresveratrol-3-O-glucoside reduced the pigmentation in guinea pig skin. These compounds suppressed the expression of melanogenic enzymes tyrosinase, tyrosinase-related protein-1, and microphthalmia transcription factor. The anti-melanogenesis effect was highest with oxyresveratrol, intermediate with oxyresveratrol-3-O-glucoside, and lowest with mulberroside A. Mulberroside A is a glycosylated stilbene of oxyresveratrol; thus, the deglycosylation of mulberroside A resulted in enhanced inhibition of melanogenesis. Histological analysis with Fontana-Masson staining confirmed that these compounds significantly reduced the melanin content in the epidermis of UVB-irradiated guinea pig skin compared to the vehicle control. Thus, these compounds effectively reduced pigmentation and may be suitable cosmetic agents for skin whitening. (C) 2011 Elsevier Ltd. All rights reserved.