Immunohistochemical survey of p16INK4A expression in normal human adult and infant tissues.
Immunohistochemical survey of p16INK4A expression in normal human adult and infant tissues.
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发表时间：
1999-09
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通讯作者：
Gunnlaugur P. Nielsen;A. Stemmer-Rachamimov;J. Shaw;Jennifer E. Roy;J. Koh;David N. Louis
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作者：
Gunnlaugur P. Nielsen;A. Stemmer-Rachamimov;J. Shaw;Jennifer E. Roy;J. Koh;David N. Louis
p16INK4A是一种细胞周期抑制剂，通常在人类肿瘤和肿瘤细胞系中失活。尽管它在人类肿瘤中的重要性，p16表达的正常模式在很大程度上仍然未知。因此，我们分析了p16在所有人体器官中的免疫组织化学定位，并证明细胞p16表达是高度选择性的。在成人中，增殖期子宫内膜、乳腺导管上皮、宫颈鳞状上皮和输卵管化生上皮、食管鳞状上皮、唾液腺和胃窦腺均强烈表达该蛋白。p16还广泛表达于内分泌腺，包括胰腺中的朗格汉斯细胞和睾丸中的前垂体细胞以及Leydig和Sertoli细胞。然而，在每个组织中，p16的表达与细胞增殖或成熟无关。在婴儿中，p16染色仅限于胸腺Hassall小体，偶尔胸腺淋巴细胞，只有罕见的胰腺上皮细胞。因此，p16在成人组织中的表达增加，如在小鼠组织中，可能反映了p16在细胞衰老中的作用。限制p16在婴儿胸腺中的表达，胸腺是唯一致力于早期衰老的器官，也与这种作用相一致。正常组织中p16表达模式的记录将有助于我们了解该蛋白的正常功能，并有助于解释人类肿瘤中潜在改变的p16表达。
p16INK4A is a cell cycle inhibitor that is commonly inactivated in human tumors and tumor cell lines. Despite its importance in human neoplasia, the normal pattern of p16 expression remains largely unknown. Therefore, we analyzed the immunohistochemical localization of p16 in all human organs and demonstrated that cellular p16 expression is highly selective. In adults, proliferative endometrium, breast ductal epithelium, squamous and tubal metaplastic epithelium of the uterine cervix, esophageal squamous epithelium, salivary glands, and antral gastric glands all strongly express the protein. p16 is also widely expressed in endocrine glands, including Langerhans cells in the pancreas and anterior pituicytes and Leydig and Sertoli cells in testis. Within each tissue, however, p16 expression does not correlate with cellular proliferation or maturation. In infants, p16 staining was limited to thymic Hassall's corpuscles, occasional thymic lymphocytes, and only rare pancreatic epithelial cells. Therefore, increased expression of p16 in adult tissues, as in mouse tissues, may reflect a role of p16 in cellular senescence. Restriction of p16 expression in infants to the thymus, the only organ committed to early senescence, is also consistent with such a role. Documentation of the pattern of p16 expression in normal tissues will contribute to our understanding of the normal function of this protein and to interpretation of potentially altered p16 expression in human tumors.