RIBEYE, a component of synaptic ribbons:: A protein's journey through evolution provides insight into synaptic ribbon function
RIBEYE, a component of synaptic ribbons:: A protein's journey through evolution provides insight into synaptic ribbon function
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DOI：
10.1016/s0896-6273(00)00159-8
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发表时间：
2000-12-01
期刊：
影响因子：
16.2
通讯作者：
Südhof, TC
中科院分区：
文献类型：
--
作者：
Schmitz, F;Königstorfer, A;Südhof, TC
光感受器细胞利用带状突触以高分辨率传递感觉信号。带状突触紧张性地释放神经递质，通过突触囊泡在突触前带状物上的连续对接，使得高释放速率成为可能。我们从视网膜中部分纯化了突触带，并鉴定了一种称为RIBEYE的主要蛋白质组分。RIBEYE是由一个独特的A结构域特异性的丝带，和B结构域相同的CtBP 2，转录阻遏物，反过来是有关的2-羟酸脱氢酶。A结构域介导RIBEYE组装成大结构，而B结构域以高亲和力结合NAD（+），类似于2-羟酸脱氢酶。我们的研究结果定义了一个独特的组件突触带，并建议RIBEYE进化脊椎动物利用预先存在的蛋白质，建立一个独特的支架专门的突触。
Photoreceptor cells utilize ribbon synapses to transmit sensory signals at high resolution. Ribbon synapses release neurotransmitters tonically, with a high release rate made possible by continuous docking of synaptic vesicles on presynaptic ribbons. We have partially purified synaptic ribbons from retina and identified a major protein component called RIBEYE. RIBEYE is composed of a unique A domain specific for ribbons, and a B domain identical with CtBP2, a transcriptional repressor that in turn is related to 2-hydroxyacid dehydrogenases. The A domain mediates assembly of RIBEYE into large structures, whereas the B domain binds NAD(+) with high affinity, similar to 2-hydroxyacid dehydrogenases. Our results define a unique component of synaptic ribbons and suggest that RIBEYE evolved in vertebrates under utilization of a preexisting protein to build a unique scaffold for a specialized synapse.