Generation of mutation hotspots in ageing bacterial colonies.
Generation of mutation hotspots in ageing bacterial colonies.
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DOI：
10.1038/s41598-016-0005-4
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发表时间：
2016-12-05
影响因子：
4.6
通讯作者：
Danchin A
中科院分区：
文献类型：
--
作者：
Sekowska A;Wendel S;Fischer EC;Nørholm MHH;Danchin A
老化的细菌菌落如何产生适应性突变体？在两个月的时间里，我们分离出了能够使用新碳源的老化菌落，并对它们的基因组进行了测序。这使我们能够揭示其适应背后的分子机制的精致细节：大多数突变位于基因组中的几个热点，随着时间的推移，突变越来越多地与8-氧代鸟苷的参与一致，仅在转录链上形成。这项工作提供了强有力的支持，作为一个通用的过程，在老化过程中产生适应性突变的逆转突变。
How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.