Histologic features of venous invasion, expression of vascular endothelial growth factor and matrix metalloproteinase-2 and matrix metalloproteinase-9, and the relation with liver metastasis in pancreatic cancer
Histologic features of venous invasion, expression of vascular endothelial growth factor and matrix metalloproteinase-2 and matrix metalloproteinase-9, and the relation with liver metastasis in pancreatic cancer
复制标题
DOI：
10.1097/00006676-200203000-00008
复制
发表时间：
2002-03-01
期刊：
影响因子：
2.9
通讯作者：
Koyanagi, Y
中科院分区：
文献类型：
--
作者：
Nagakawa, Y;Aoki, T;Koyanagi, Y
简介：胰腺癌常伴有静脉浸润和血行转移，目的：探讨胰腺癌受侵静脉的形态学特征、肿瘤内血管成分、与肝转移的关系、血管内皮生长因子（VEGF）、基质金属蛋白酶（MMP）-2和MMP-9的表达及血行转移的发生机制。术后肝转移18例，无肝转移14例。通过维多利亚蓝和CD 34染色检查标本以确定静脉和微血管的组成。结果：32例患者中31例有静脉浸润，31例有血管内皮生长因子（VEGF）、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）表达。肝转移组中、大静脉浸润密度明显高于非肝转移组，且与MMP-2、MMP-9的过度表达有关。被侵犯的静脉与破碎的管腔通过癌细胞被认为是一个渗透的癌症（破坏型静脉），其数量与肝转移及MMP-2、MMP-9过表达显著相关。总之，几乎所有的胰腺癌患者都有静脉侵犯，提示浸润大静脉和破坏型静脉可能是肝转移的危险因素，MMP-2和MMP-9表达增高与肝转移有关。
Introduction: Pancreatic cancer frequently is associated with venous invasion and hematogenous metastasis.Aims: To determine morphologic features of invaded veins, intratumoral vascular composition, the correlation with liver metastasis, and expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2 and MMP-9, and the mechanism of development of hematogenous metastasis.Methodology: We examined 32 patients with resected pancreatic cancer: 18 had postoperative liver metastasis, and 14 had no liver metastasis. Specimens were examined to determine the composition of veins and microvessels by staining of victoria-blue and CD34. We also investigated expression of VEGF, MMP-2, and MMP-9 by immunohistochemical staining.Results: Venous invasion was detected in 31 of 32 patients. Invaded venous densities of middle- and large-sized veins were significantly higher in patients with liver metastasis than in those with nonliver metastasis, and they were related to MMP-2 and MMP-9 overexpression. Invaded veins with fragmentation of the lumen through cancer cells were considered to be an intravasation of cancer (destroyed type vein), and their numbers were significantly related to liver metastasis, and MMP-2 and MMP-9 overexpression.Conclusion: In conclusion, almost all the patients with pancreatic cancer showed venous invasion, indicating that invasion into large veins and destroyed type veins could be a risk factor for liver metastasis and that increased expression MMP-2 and MMP-9 were related to such invasion.