MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR (Retracted article. See April, 2017)
MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR (Retracted article. See April, 2017)
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DOI：
10.1007/s13277-014-2342-x
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发表时间：
2014-11-01
期刊：
影响因子：
--
通讯作者：
Fang, Long
中科院分区：
文献类型：
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作者：
Duan, Jian-Hua;Fang, Long
MicroRNAs(MiRNAs)是一类非编码的小RNA，通过下调多种靶基因在人类肿瘤发生中发挥重要作用。在本研究中，我们发现miR-92在胃癌组织中表达上调，而在癌旁正常组织中表达上调。有趣的是，miR-92的表达与患者的临床特征显著相关。体外功能得失实验进一步表明miR-92模拟物明显促进胃癌细胞的增殖和侵袭，而其反义寡核苷酸则抑制胃癌细胞的增殖和侵袭。此外，荧光素酶报告分析和Western印迹表明，法尼类X受体(FXR)是miR-92的直接靶标。因此，我们的数据提示miR-92的上调可能是胃癌发生的一个重要机制。
MicroRNAs (miRNAs), a class of small noncoding RNAs, play critical roles in human carcinogenesis through downregulation of various target genes. In the present study, we found that miR-92 is upregulated in gastric cancer tissues compared with adjacent normal tissues. Interestingly, miR-92 expression is significantly associated with clinical characteristics of patients. Gain or loss-of-function in vitro experiments further show that miR-92 mimics significantly promoted, while its antisense oligos inhibited gastric cancer cell proliferation and invasion. Moreover, luciferase reporter assays and western blot indicated that farnesoid X receptor (FXR), is a direct target of miR-92. Therefore, our data suggest that upregulation of miR-92 may represent an important mechanism for the development of gastric cancer.