High throughput screening of methylation status of genes in prostate cancer using an oligonucleotide methylation array.
High throughput screening of methylation status of genes in prostate cancer using an oligonucleotide methylation array.
复制标题
使用寡核苷酸甲基化阵列高通量筛选前列腺癌基因的甲基化状态。
DOI：
10.1093/carcin/bgh310
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Luo,Jian-Hua
中科院分区：
文献类型：
--
作者：
Yu,YanPing;Paranjpe,Shirish;Nelson,Joel;Finkelstein,Sydney;Ren,Baoguo;Kokkinakis,Demetrius;Michalopoulos,George;Luo,Jian-Hua
最近的工作使用高通量微阵列技术已经发现了前列腺癌中大量基因的表达改变。这些改变中的许多可能是这些基因的启动子或外显子1区域的CpG岛中甲基化状态变化的结果。为了检测大量基因和EST的甲基化状态，我们将匹配/错配杂交原理与全基因组标记技术相结合，建立了一种高特异性的基于寡核苷酸的甲基化微阵列。使用该阵列，我们分析了三种前列腺癌细胞系中105个基因和EST的甲基化状态。在这些细胞系中，这些基因和EST中有32%至47%被甲基化。通过将该阵列的甲基化状态与三种前列腺癌细胞系的Affyphin表达阵列的结果相关联，我们确定基因的甲基化在下调前列腺癌中某些基因的表达中起着重要作用（37%）。我们还在许多原发性前列腺组织样本上测试了该阵列。我们的研究结果表明，在这个微阵列中的一个基因子集（25/105）在所有前列腺癌样本中甲基化，但在正常前列腺中没有甲基化，这表明某些基因的甲基化状态的改变在前列腺癌的发展中具有潜在的意义。
Recent work using high-throughput microarray technology has discovered altered expression of a large number of genes in prostate cancer. Many of these alterations may be the consequence of changes in methylation status in the CpG islands of promoter or exon 1 regions of these genes. In order to determine the methylation status of a large number of genes and ESTs we combined the principle of match/mismatch hybridization with the technique of whole genome labeling to develop a highly specific oligonucleotide-based methylation microarray. Using this array, we analyzed the methylation status of 105 genes and ESTs in three prostate cancer cell lines. Between 32 and 47% of these genes and ESTs were methylated in these cell lines. By correlating the methylation status of this array with the results of Affymetrix expression arrays of three prostate cancer cell lines, we determined that methylation of genes played a significant role (37%) in down-regulating the expression of certain genes in prostate cancer. We also tested this array on a number of primary prostate tissue samples. Our results indicated that a subset of genes in this microarray (25/105) were methylated in all prostate cancer samples but not in normal prostate, suggesting the potential significance of alterations in the methylation status of certain genes in the development of prostate cancer.
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook