Clinical significance of RUNX2 expression in patients with nonsmall cell lung cancer: a 5-year follow-up study
Clinical significance of RUNX2 expression in patients with nonsmall cell lung cancer: a 5-year follow-up study
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非小细胞肺癌患者RUNX2表达的临床意义：一项5年随访研究
DOI：
10.1007/s13277-013-0720-4
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发表时间：
2013-06-01
期刊：
影响因子：
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通讯作者：
Xu, Jian-Ping
中科院分区：
文献类型：
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作者：
Li, Hong;Zhou, Ren-Jie;Xu, Jian-Ping
本研究旨在探讨矮小相关转录因子(RUNX)-2在非小细胞肺癌(NSCLC)中的表达及其预后意义。采用实时定量逆转录聚合酶链式反应(qRT-PCR)和免疫印迹法检测非小细胞肺癌组织和癌旁正常组织中Runx2基因的表达。另外，采用免疫组织化学方法检测121例临床病理特征的非小细胞肺癌组织中RUNX2的表达。统计分析RUNX2的表达与临床病理特征及预后的关系。定量逆转录聚合酶链式反应和免疫印迹结果均显示，非小细胞肺癌组织中RUNX2的mRNA和蛋白水平均显著高于同一个体的癌旁正常组织。免疫组织化学分析显示，RUNX2的表达与肿瘤大小、肿瘤分期、有无淋巴结转移密切相关。Kaplan-Meier法显示RUNX2高表达与NSCLC患者术后生存时间短相关，Cox回归分析发现RUNX2表达是影响NSCLC患者术后生存时间的独立危险因素。综上所述，RUNX2可能在非小细胞肺癌的发生发展中起重要作用，RUNX2可能作为一种新的预后标志物。
This study was designed to evaluate expression and prognostic significance of runt-related transcription factor (RUNX)-2 in human nonsmall cell lung cancer (NSCLC). RUNX2 expression was examined at mRNA and protein levels by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot in NSCLC tissues and adjacent normal tissues. In addition, RUNX2 expression was analyzed by immunohistochemistry in 121 clinicopathologically characterized NSCLC cases. The relationship between the expression of RUNX2 and clinicopathological characteristics and prognosis was statistically analyzed. Both qRT-PCR and Western blot demonstrated that RUNX2 mRNA and protein levels were significantly higher in NSCLC tissues compared to the adjacent normal tissues from the same individual. Immunohistochemistry analysis showed that RUNX2 expression was significantly correlated with tumor size, tumor stage, and lymph node metastasis. Higher RUNX2 expression was associated with shorter postoperative survival time of NSCLC patients by Kaplan–Meier method and was found to be an independent risk factor that influences the postoperative survival time of NSCLC patients by Cox regression analysis. In conclusion, these data showed that RUNX2 may play an important role in NSCLC tumorigenesis, and RUNX2 might serve as a novel prognostic marker in NSCLC.