Disease-specific recombinant allergens for the diagnosis of allergic bronchopulmonary aspergillosis
Disease-specific recombinant allergens for the diagnosis of allergic bronchopulmonary aspergillosis
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DOI：
10.1093/intimm/10.8.1211
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发表时间：
1998-08-01
影响因子：
4.4
通讯作者：
Blaser, K
中科院分区：
文献类型：
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作者：
Crameri, R;Hemmann, S;Blaser, K
过敏性支气管肺曲霉病（ABPA）是由烟曲霉菌引起的严重肺部并发症，是一种复杂的临床综合征，具有明确的血清学、病理学和临床特征。ABPA的诊断通常是困难的，因为一些重叠的临床和实验室检查结果之间共享哮喘与致敏A，烟曲霉和ABPA，但必要的治疗，以防止肺功能的严重恶化。我们从噬菌体表面展示的cDNA文库中克隆了烟曲霉变应原基因，测序并在大肠杆菌中产生重组蛋白。用单个重组变应原评估了烟曲霉致敏哮喘患者（含或不含ABPA）和健康对照组的免疫应答。18.5kDa过氧化物酶体蛋白和23kDa锰超氧化物歧化酶rAspf6被鉴定为具有已知生物学功能的蛋白质，而30kDa变应原rAspf4与已知蛋白质缺乏序列同源性。分泌的核糖毒素rAspf1和rAspf3可被A.有或没有ABPA的烟致敏哮喘患者，而非分泌型锰超氧化物歧化酶rAsp f 6和rAsp f 4变应原仅被ABPA患者的血清IgE识别。对单个重组A.哮喘患者中的烟曲霉变应原允许区分ABPA和A.特异性为100%，敏感性为90%。
Allergic bronchopulmonary aspergillosis (ABPA), a severe pulmonary complication caused by Aspergillus fumigatus, is considered a complex clinical syndrome with defined serological, pathological radiological and clinical features. The diagnosis of ABPA is often difficult because of several overlapping clinical and laboratory findings shared between asthma with sensitization to A, fumigatus and ABPA, but essential for treatment to prevent severe deterioration of pulmonary function. We have cloned A, fumigatus allergens from a cDNA library displayed on phage surface, sequenced the inserts and produced recombinant proteins in Escherichia coli, The single recombinant allergens were used to assess the immunological response in representative groups of A, fumigatus-sensitized asthmatics with or without ABPA and healthy controls, The allergens rAsp f 1, a 16.9 kDa ribotoxin, rAsp f 3, a 18.5 kDa peroxisomal protein, and rAsp f 6, a 23 kDa manganese superoxide dismutase, were identified as proteins with known biological function and rAsp f 4, a 30 kDa allergen, lacks sequence homology to known proteins. The secreted ribotoxin rAsp f 1 and rAsp f 3 are recognized by serum IgE of A. fumigatus-sensitized asthmatics with or without ABPA, whereas the non-secreted manganese superoxide dismutase rAsp f 6 and the rAsp f 4 allergen are exclusively recognized by serum IgE of ABPA patients. The dissection of IgE-mediated immune responses to single recombinant A. fumigatus allergens in asthmatic patients allow a discrimination between ABPA and A. fumigatus sensitization with high specificity (100%) and sensitivity (90%).