Isolation and Partial Sequence of the A‐Protein Gene of Thermus thermophilus Cytochrome c1aa3 a
Isolation and Partial Sequence of the A‐Protein Gene of Thermus thermophilus Cytochrome c1aa3 a
复制标题
嗜热栖热菌细胞色素 c1aa3 a 的 A 蛋白基因的分离和部分序列
DOI：
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发表时间：
1988
影响因子：
5.2
通讯作者：
G. Buse
中科院分区：
文献类型：
--
作者：
J. Fee;M. W. Mather;P. Springer;S. Hensel;G. Buse
Thermus thermophilus is a strictly aerobic eubacterium that grows optimally near 70°C. Its respiratory system is very similar to that of eukaryotic mitochondria, and the organism has proven to be a particularly good source of stable, comparatively simple respiratory enzymes [cf. Refs. 1 and 21. There are a t least two terminal oxidases: The recently discovered cytochrome ba: and cytochrome c,aa3? Cytochrome ba, is an analog of aa, in which the heme A of cytochrome a is replaced with protoporphyrin IX (heme B) while its other redox components appear to be largely identical to those of the now classical mammalian cytochrome aa, [cf. Zimmerman et ~ 1 . ~ 1 ; it has only a single 35-kDa protein subunit. Cytochrome qua3 consists of two polypeptides. The -33-kDa C-protein covalently binds one heme C, while the -55-kDa protein is thought to bind the four canonical redox centers of aa,, two heme A, and two Cu [cf. Refs. 1 and 21. Toward our goal of unequivocally establishing the distribution of the metal centers in cytochrome c,aa,, we have isolated the structural gene of the A-protein.
干琼脂糖凝胶中碱基组成独立的杂交：基因组 DNA 片段克隆的筛选和回收。
影响因子：
2.7
作者：
Mather,MW
通讯作者：
Mather,MW
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yoshida,T;Lorence,RM;Choc,MG;Tarr,GE;Findling,KL;Fee,JA
通讯作者：
Fee,JA
来自极端嗜热需氧细菌的呼吸蛋白。
DOI：
10.1016/0304-4173(86)90009-1
发表时间：
1986
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Fee,JA;Kuila,D;Mather,MW;Yoshida,T
通讯作者：
Yoshida,T